Product nameMouse Heme Oxygenase 1 Matched Antibody Pair Kit
See all Heme Oxygenase 1 kits
Assay typeELISA set
Range62.5 pg/ml - 4000 pg/ml
Species reactivityReacts with: Mouse
Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant Heme Oxygenase 1. Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab204524) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
The capture antibody is glycerol free.
The detector antibody contains glycerol.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 2 x 96 tests Heme Oxygenase 1 Capture Antibody 1 x 100µg 1 x 20µg Heme Oxygenase 1 Detector Antibody 1 x 25µg 1 x 5µg Heme Oxygenase 1 Lyophilized Protein 1 vial 1 vial
FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
Sequence similaritiesBelongs to the heme oxygenase family.
Cellular localizationMicrosome. Endoplasmic reticulum.
- Information by UniProt
- 32 kD
ab213737 has not yet been referenced specifically in any publications.