Product nameMouse Hemoglobin subunit alpha Matched Antibody Pair Kit
See all Hemoglobin subunit alpha kits
Assay typeELISA set
Range781.25 pg/ml - 50000 pg/ml
Species reactivityReacts with: Mouse
Mouse Hemoglobin subunit alpha Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Hemoglobin subunit alpha.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
We’ve listened to you: due to popular demand, we will now provide our Matched Antibody Pair kits in 5x96 tests and 10x96 tests. The 2x96 tests size will be discontinued on 30th June 2020 unless inventory is depleted beforehand.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 10 x 96 tests 5 x 96 tests Mouse Hemoglobin subunit alpha Capture Antibody 1 x 100µg 1 x 50µg Mouse Hemoglobin subunit alpha Detector Antibody 1 x 25µg 1 x 12.5µg Mouse Hemoglobin subunit alpha Lyophilized Protein 1 vial 1 vial
FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
Tissue specificityRed blood cells.
Involvement in diseaseHeinz body anemias
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease
Sequence similaritiesBelongs to the globin family.
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated.
- Information by UniProt
- Alpha 1 globin
- Alpha globin
- Alpha one globin
Our Abpromise guarantee covers the use of ab222282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab222282 has not yet been referenced specifically in any publications.