Overview

  • Product name

    Mouse Hemopexin ELISA Kit (HPX)
    See all Hemopexin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Serum, Plasma, Other biological fluids
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.509 ng/ml
  • Range

    12.5 ng/ml - 400 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum >=85 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Hemopexin (HPX) Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Hemopexin in biological samples of mice.


    In this assay the Hemopexin present in samples reacts with the anti-Hemopexin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- Hemopexin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hemopexin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hemopexin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hemopexin in the test sample. The quantity of Hemopexin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab157716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157716 Hemopexin Mouse ELISA Kit.

Protocols

References

This product has been referenced in:

  • Lawson HA  et al. Physiologic and genetic evidence links hemopexin to triglycerides in mice and humans. Int J Obes (Lond) 41:631-638 (2017). Read more (PubMed: 28119529) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
quality products, and easy to use

Abcam user community

Verified customer

Submitted Jan 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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