Overview

  • Product name

    Mouse HGF ELISA Kit
    See all HGF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.4 ng/ml
  • Range

    0.614 ng/ml - 60 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111.8 98% - 123%
    Serum 111.4 98% - 129%
    Plasma 105.8 80% - 120%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s HGF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HGF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse HGF coated on a 96- well plate. Standards and samples are pipetted into the wells and HGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse HGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100686

  • Representative Standard Curve using ab100686

Protocols

References

This product has been referenced in:

  • Boldyreva MA  et al. Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 31238604) »
  • El Ouaamari A  et al. Liver-derived systemic factors drive ß cell hyperplasia in insulin-resistant states. Cell Rep 3:401-10 (2013). Read more (PubMed: 23375376) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Question
Answer

The antibody pairs used in this kit recognize both the alpha and beta chains of mouse HGF

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Question
Answer

The antibody pair inab100686 recognizes an epitope located somewhere between aa 33-728 (which is whole sequence minus the signal peptide). Therefore the kit is expected to detect the pro (uncleaved) form, the heterodimeric form, the alpha subunit, and the beta subunit (so total HGF). Please note that we have not specifically tested each form separately but both antibodies are polyclonal so it is likely.

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Question
Answer

Thank you for contacting us.

Both mouse and human HGF ELISA kits will detect total HGF.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your calls this week and for your patience.

I've heard back from my contact at the lab, and the standard is biologically active. Activity was measured as the ability to stimulate H-thymidine incorporation in 4MBr-5 monkey cells. The ED50 is typically 20-40 ng/mL.

I hope this information will be useful, but please let me know if you need anything else and I'll be happy to help.

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Answer

Thank you for contacting Abcam. Unfortunately, epitope mapping has not been performed for this kit so we do not know exactly where the antibodies are binding.  Therefore whether the kit will recognize HGF bound to the c-met receptor or inhibit c-met from binding, is not known.   I wish I could be of more help on this one. If there is anything else I can help you with, please let me know.

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Question
Answer

The best kit to use for tissue samples is ab100687 HGF Mouse ELISA Kit - 1 x 96 Well Plate. This is specifically meant for tissue samples. Both of the antibodies in the kit are polyclonal and considering that the homology between mouse and rat is high (around 98%), the odds are high that these kits would be suitable for rat samples. However, we have not tested this and are unable to give guarantee.

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