Overview

  • Product name

    Mouse HGF ELISA Kit
    See all HGF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.9%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    8.4 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111 109% - 117%
    Cell culture extracts 104 101% - 107%
    Tissue Extracts 119 113% - 123%
    Cell culture media 109 107% - 112%
    EDTA Plasma 104 100% - 108%
    Citrate Plasma 108 105% - 111%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Human
  • Product overview

    HGF in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of HGF protein in mouse  serum, plasma, cell culture supernatant, cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Hepatocyte Growth Factor (HGF) is mitogen of parenchymal hepatocytes.  HGF also acts as a growth factor and is expressed by a wide variety of tissue and cell types.    HGF binds to the HGF- Receptor to help regulate cell growth, cell motility and morphogenesis.  Elevated circulating levels of HGF can be measured as prognostic marker of severity of hypertension and heart failure.  HGF is secreted as a full length inactive polypeptide, and then is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta chain which dimerize producing the active heterodimeric molecule.  Mouse HGF SimpleStep ELISA® (ab223862) is designed to recognize this dimerized protein.


    The calculated minimal detectable dose (MDD) is 8.4 pg/mL in Sample Diluent NS.


    The calculated minimal detectable dose (MDD) is 11 pg/mL in 1X Cell Extraction Buffer PTR.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 12ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    10X Mouse HGF Capture Antibody 1 x 600µl
    10X Mouse HGF Detector Antibody 1 x 600µl
    Mouse HGF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
  • Involvement in disease

    Deafness, autosomal recessive, 39
  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Alternative names

    • DFNB39
    • F TCF
    • Fibroblast derived tumor cytotoxic factor
    • Hepatocyte growth factor
    • Hepatocyte growth factor (hepapoietin A; scatter factor)
    • Hepatocyte growth factor beta chain
    • Hepatocyte growth factor precursor
    • Hepatopoietin A
    • Hepatopoietin-A
    • Hgf
    • HGF_HUMAN
    • HGFB
    • HPTA
    • Lung fibroblast derived mitogen
    • OTTHUMP00000161349
    • OTTHUMP00000206710
    • OTTHUMP00000206711
    • OTTHUMP00000206712
    • OTTHUMP00000206713
    • OTTHUMP00000206730
    • Scatter factor
    • SF
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab223862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse HGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (EDTA) 50%, plasma (citrate) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 11,764 pg/mL in serum, 1099 pg/mL in plasma (EDTA), 691 pg/mL in plasma (citrate) and 896 pg/mL in plasma heparin).

  • The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 7,859 pg/mL in NIH/3T3 cell extract, 2,472 pg/mL in mouse spleen tissue extract and 1,291 pg/mL in mouse kidney tissue extract.

  • Mouse 3T3-L1 cells were either undifferentiated (not allowed to reach confluency) or differentiated (cultured for 48 hours in adipocyte differentiation medium).  The concentrations of HGF were measured in duplicate and interpolated from the HGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 2,636 pg/mL in undifferentiated 3T3-L1 cell extract and 88 pg/mL in adipocyte differentiated 3T3-L1 cell extract.

  • The concentrations of HGF were measured in duplicates, interpolated from the HGF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (EDTA) 100%, plasma (citrate) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HGF concentration was determined to be 79,543 pg/mL in serum, 3,151 pg/mL in plasma (EDTA), 2,395 pg/mL in plasma (citrate) and 2,611 pg/mL in plasma heparin).

  • Recombinant rat HGF was prepared at 4 ng/mL and diluted with a 2-fold titration alongside of mouse HGF standard curve, and assayed for cross reactivity. 100% cross-reactivity was observed.

Protocols

References

ab223862 has not yet been referenced specifically in any publications.

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