Overview

  • Product name
    Mouse Anti-Human IgG4 pFc’ [HP6023] (HRP)
    See all IgG4 secondary antibodies
  • Host species
    Mouse
  • Target species
    Human
  • Tested applications
    Suitable for: ICC, ELISA, Dot blot, WB, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG4.

  • Conjugation
    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab99817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/1000 - 1/2000.
Dot blot Use at an assay dependent dilution. for Dot and slot immunoblotting.
WB Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Images

  • ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99817.

References

This product has been referenced in:
  • Perugino CA  et al. Identification of galectin-3 as an autoantigen in patients with IgG4-related disease. J Allergy Clin Immunol N/A:N/A (2018). ELISA . Read more (PubMed: 29852256) »
  • Campa MJ  et al. Complement Factor H Antibodies from Lung Cancer Patients Induce Complement-Dependent Lysis of Tumor Cells, Suggesting a Novel Immunotherapeutic Strategy. Cancer Immunol Res 3:1325-32 (2015). ELISA . Read more (PubMed: 26216416) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


ab99817 has been tested by ELISA against purified Human IgG1, IgG2, IgG3, and IgG4 myelomas. Additionally, we see minimal cross reactivity with the other sub-isotypes in-house.

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Answer

Following on my previous message about the secondary antibodies you are interested in, the anit-human IgG isotypes are tested on a Human IgG subclass plate (IgG1, IgG2, IgG3, and IgG4) to ensure activity and minimal cross reactivity with the other IgG subclasses.

mouse mAb anti human IgG1-HRP #ab99774

mouse mAb anti human IgG2-HRP #ab99784

mouse mAb anti human IgG3-HRP #ab99829

mouse mAb anti human IgG4-HRP #ab99817



Please let me know if you need the data.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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