Overview

  • Product name

    Anti-Mouse IgE antibody
  • Description

    Goat polyclonal to Mouse IgE
  • Host species

    Goat
  • Specificity

    Reacts only with IgE by IEP, gel diffusion and ELISA.
  • Tested applications

    Suitable for: ELISA, Immunoelectrophoresismore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse Mouse IgE.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Immunoaffinity purified off an immunoabsorbant containing a mouse IgE protein different from that used for immunization.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab19967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Immunoelectrophoresis Use at an assay dependent dilution.

Target

  • Relevance

    IgE mediates the immune responses in the pathogenesis of allergic asthmas. This has been shown in the successful use of recombinant 'humanised' monoclonal antibodies against IgE in the treatment of moderate-to-severe allergic asthma where the antibody forms complexes with free IgE and blocks its interaction with mast cells and basophils.

References

This product has been referenced in:

  • Meixiong J  et al. Activation of Mast-Cell-Expressed Mas-Related G-Protein-Coupled Receptors Drives Non-histaminergic Itch. Immunity 50:1163-1171.e5 (2019). Read more (PubMed: 31027996) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam about ab19967. It has not been tested in IHC and so we cannot guarantee that it will work in this particular application. If you wish to take advantage of our testing discount program, then I would gladly send you the information on that program. If there is anything else I can help you with, please do hesitate to contact me.

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Answer

Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding ab19967. The affinity constant and the sensitivity of this IgE antibody have not yet been determined. However, this antibody has been used to capture IgE from mouse sera so we believe it has a high affinity for the IgE. The suggested starting concentration is 1.0 - 5.0 ug/mL.   I hope this information is useful, but please let me know if you have any further questions and I'll be happy to help.

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