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I plan to use IGF1 1x IGF1 Mouse ELISA Kit (ab100695) and I was wondering whether I need to use Bradford protein determination assay in order to normalize to the amount protein that I have in my serum?
Asked on Feb 13 2013
Thank you for your inquiry.
I can confirm that it is not necessary to determine the total protein in serum samples before using them with this kit. We are assuming the samples that are taken for one experiment all have roughly the same concentration of total protein.
I strongly suggest to do a serial dilution with the samples to make sure that they fall in the range of the standard curve.
I hope this information is helpful and wish you good luck with your research.
Answered on Feb 13 2013