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How likely is it that this kit will work with samples extracted with Trizol?
Asked on Jul 09 2013
It would not be recommended to use Trizol for extraction as it contains Guanidinium thiocyanate which is a strong protein denaturant. Antibodies used in this kit recognize conformational epitopes of the target protein so it is important that the proteins are not denatured and retain their native 3-D shape.
Jeremy KasanovAbcam Scientific Support
Answered on Jul 09 2013