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What levels of IGF1 did you obtain when you tested this kit with mouse plasma? I ran my mouse plasma samples and obtained a 42 pg/ml concentration of IGF1, and the literature reports 200-600 pg/ml of IGF1 in mouse plasma. I performed an overnight initial binding incubation on an orbital rocker.
Additionally, I did have to transport my samples from a different facility on ice to my laboratory for testing on the same day. During this transportation process, would you recommend adding protease inhibitors directly to the mouse plasma after I draw it? Any other advice would be much appreciated.
Asked on Feb 26 2014
We tested normal mouse serum/plasma levels ˜300 ng/ml (Serum/plasma-EDTA), ˜30ng/ml( plasma-Citrate and heparin).
IGF1 stability depends on how long they will be in travel. If it is a short period of time (1-2 hrs or so) ice would be sufficient but if it will be a longer period of time, you may want to add protease inhibitors.
What type of anti-coagulant do you have in your plasma? As you can see above, the laboratory only obtained values of 30 ng/ml (close to your values) with mouse plasma with citrate and heparin. Higher values (300 ng/ml) closer to those reported in the paper you referenced were obtained when the lab tested serum/plasma with EDTA. The paper that you provided used serum, so this may be the difference between your samples and those reported in the paper.
Kevin HansonAbcam Scientific Support
Answered on Feb 26 2014