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Signal Transduction Signaling Pathway Lipid Signaling Other
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Mouse IGFBP-1 ELISA Kit (ab213865)

  • Datasheet
  • SDS
  • Protocol Booklet
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Mouse IGFBP-1 ELISA Kit (ab213865) Standard Curve

    Key features and details

    • Sensitivity: 3 pg/ml
    • Range: 31.2 pg/ml - 2000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    Overview

    • Product name

      Mouse IGFBP-1 ELISA Kit
      See all IGFBP1 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 88pg/ml 6.6 7.5%
      2 16 281pg/ml 14.77 5.4%
      3 16 865pg/ml 38.06 4.4%
      Inter-assay
      Sample n Mean SD CV%
      1 24 90pg/ml 7.92 8.8%
      2 24 267pg/ml 19.49 7.3%
      3 24 783pg/ml 36.8 4.7%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 3 pg/ml
    • Range

      31.2 pg/ml - 2000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      The Mouse IGFBP-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213865) is designed for the quantitative detection of Mouse IGFBP-1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from goat specific for IGFBP-1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A26-N272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse IGFBP-1 amount of sample captured in plate. The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27 KDa.

    • Notes

      IGFBP-1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Mouse IGFBP-1 coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Mouse IGFBP-1 antibody 1 x 130µl
      Lyophilized recombinant Mouse IGFBP-1 standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Other
      • Signal Transduction
      • Growth Factors/Hormones
      • Insulin / Insulin-like
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
    • Function

      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
    • Sequence similarities

      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational
      modifications

      Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P08833 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • AFBP
      • Alpha pregnancy associated endometrial globulin
      • Amniotic fluid binding protein
      • Binding protein 25
      • Binding protein 26
      • Binding protein 28
      • Growth hormone independent binding protein
      • hIGFBP 1
      • hIGFBP1
      • IBP 1
      • IBP-1
      • IBP1
      • IBP1_HUMAN
      • IGF binding protein 1
      • IGF BP25
      • IGF-binding protein 1
      • IGFBP 1
      • IGFBP-1
      • IGFBP1
      • Insulin like growth factor binding protein 1
      • Insulin-like growth factor-binding protein 1
      • Placental protein 12
      • PP 12
      • PP12
      see all
    • Database links

      • Entrez Gene: 16006 Mouse
      • SwissProt: P47876 Mouse
      • Unigene: 21300 Mouse

      Images

      • Mouse IGFBP-1 ELISA Kit (ab213865) Standard Curve
        Mouse IGFBP-1 ELISA Kit (ab213865) Standard Curve

        Mouse IGFBP-1 ELISA Kit (ab213865) Standard Curve

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab213865? Please let us know so that we can cite the reference in this datasheet.

      ab213865 has been referenced in 1 publication.

      • Haywood NJ  et al. IGFBP-1 in Cardiometabolic Pathophysiology-Insights From Loss-of-Function and Gain-of-Function Studies in Male Mice. J Endocr Soc 4:bvz006 (2020). PubMed: 32190801

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