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|Sample type||Average %||Range|
|Cell culture supernatant||105.5||93% - 112%|
|Serum||85.83||73% - 94%|
|Plasma||76.81||71% - 84%|
Abcam’s IGFBP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGFBP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Mouse IGFBP3 (lyophilized)||2 vials|
|IGFBP3 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IGFBP3 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
IGFBP3 in mouse tissue lysates extracted with RIPA buffer. Data from kidney lysates diluted 1/50-1/250 (protein concentration 0.25-0.05 mg x mL-1) and lung diluted 1/10 (0.75 mg x mL-1) (duplicates +/- SD).
IGFBP3 in mouse biological fluids (duplicates +/- SD). Plasma and serum tested in the dilution range of 1/50-1/250, urine tested undiluted, rat and human samples (serum and plasma) were below level of detection at 1/10 dilution.
Representative Standard Curve using ab100692
ab100692 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"