Key features and details
- Sensitivity: 5 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse IGFBP5 ELISA Kit
See all IGFBP5 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range4.12 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 91.26 80% - 101% Serum 115.3 86% - 130% Plasma 95.11 83% - 102%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s IGFBP5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse IGFBP5 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get better reproducibility in only 90 minutes with Mouse IGFBP5 ELISA Kit (ab208345) from our SimpleStep ELISA® range.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Mouse IGFBP5 2 vials IGFBP5 Microplate (12 x 8 wells) 1 unit Recombinant Mouse IGFBP5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Tissue specificityOsteosarcoma, and at lower levels in liver, kidney and brain.
Sequence similaritiesContains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain.
- Information by UniProt
- IBP 5
SimpleStep ELISA kits
ab100693 has been referenced in 1 publication.
- Vijayan A et al. IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFß1-induced mesenchymal invasion. Int J Biochem Cell Biol 45:2774-85 (2013). PubMed: 24120850