Overview

  • Product name

    Anti-Mouse IgG antibody [RM104]
    See all Mouse IgG primary antibodies
  • Description

    Rabbit monoclonal [RM104] to Mouse IgG
  • Host species

    Rabbit
  • Specificity

    This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG and rat IgG. It may cross react to goat IgG. The Fc region of this antibody has been engineered to eliminate the binding of Fc receptor.
  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse IgG .
    Database link: 16017

  • Positive control

    • Mouse IgG

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    RM104
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 38 kDa.

nonreduced

ELISA Use a concentration of 0.005 - 0.2 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance

    IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Constant region of heavy chain of IgG1 antibody
    • G1m marker antibody
    • Gm marker antibody
    • Ig gamma 1 chain C region antibody
    • Immunoglobin heavy constant gamma 1 antibody
    • Immunoglobulin gamma 1 antibody
    see all

Images

  • All lanes : Anti-Mouse IgG antibody [RM104] (ab190475) at 0.2 µg/ml

    Lane 1 : Mouse IgG1 (non-reduced)
    Lane 2 : Mouse IgG1 (reduced)
    Lane 3 : Mouse IgG2a (non-reduced)
    Lane 4 : Mouse IgG2a (reduced)
    Lane 5 : Mouse IgG2b (non-reduced)
    Lane 6 : Mouse IgG2b (reduced)
    Lane 7 : Mouse IgG3 (non-reduced)
    Lane 8 : Mouse IgG3 (reduced)
    Lane 9 : Mouse IgM (non-reduced)
    Lane 10 : Mouse IgM (reduced)
    Lane 11 : Mouse IgA (non-reduced)
    Lane 12 : Mouse IgA (reduced)
    Lane 13 : Mouse IgE (non-reduced)
    Lane 14 : Mouse IgE (reduced)

    Lysates/proteins at 0.02 µg per lane.

    Predicted band size: 38 kDa

  • ELISA titre of ab190475 mouse IgG. Plate was coated with 2 - 0.05 µg mouse IgG and a serial dilution of ab190475 (0.2-0.003 µg/ml) used as the primary antibody followed by alkaline phosphatase anti-rabbit IgG.

  • ELISA analysis of ab190475 at 10 - 200 ng/mL  using 50 ng/well of different immunoglobulins and an alkaline phosphatase conjugated anti-rabbit IgG.

References

This product has been referenced in:

  • Meng J  et al. USP5 promotes epithelial-mesenchymal transition by stabilizing SLUG in hepatocellular carcinoma. Theranostics 9:573-587 (2019). Read more (PubMed: 30809294) »
  • Yue H  et al. miR-212 regulated by HIF-1a promotes the progression of pancreatic cancer. Exp Ther Med 17:2359-2365 (2019). Read more (PubMed: 30867721) »
See all 12 Publications for this product

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