Recombinant

Recombinant Anti-Mouse IgG antibody [RMG07] (ab197767)

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Overview

  • Product name

    Anti-Mouse IgG antibody [RMG07]
    See all Mouse IgG primary antibodies

  • Description

    Goat monoclonal [RMG07] to Mouse IgG

  • Host species

    Goat

  • Specificity

    ab197767 reacts to Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.

  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Full length protein corresponding to Mouse Mouse IgG.

  • Positive control

    • ELISA: Mouse IgG.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RMG07

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml.
IP Use at an assay dependent concentration.
ELISA Use a concentration of 0.05 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance

    IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Constant region of heavy chain of IgG1 antibody
      • G1m marker antibody
      • Gm marker antibody
      • Ig gamma 1 chain C region antibody
      • Immunoglobin heavy constant gamma 1 antibody
      • Immunoglobulin gamma 1 antibody
      see all

    Images

    • ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)
      ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)

      A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197767 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

    • ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)
      ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)

      ELISA of mouse immunoglobulins shows ab197767 reacted to mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197767 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    References

    This product has been referenced in:

    • Otsuka Y  et al. Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice. Biochem Biophys Rep 18:100618 (2019). Read more (PubMed: 30805562) »
    • Sun XY  et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress-induced apoptosis. Mol Med Rep 13:661-8 (2016). IHC-P . Read more (PubMed: 26647757) »
    See all 3 Publications for this product

    Publishing research using ab197767? Please let us know so that we can cite the reference in this datasheet.

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