Recombinant

Recombinant Anti-Mouse IgG antibody [RMG07] (ab197767)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Goat monoclonal [RMG07] to Mouse IgG
  • Suitable for: ELISA
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Mouse IgG antibody [RMG07]
    See all Mouse IgG primary antibodies

  • Description

    Goat monoclonal [RMG07] to Mouse IgG

  • Host species

    Goat

  • Specificity

    ab197767 reacts to Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Full length protein corresponding to Mouse Mouse IgG.

  • Positive control

    • ELISA: Mouse IgG.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RMG07

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.05 - 1 µg/ml.

Target

  • Relevance

    IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • Constant region of heavy chain of IgG1 antibody
      • G1m marker antibody
      • Gm marker antibody
      • Ig gamma 1 chain C region antibody
      • Immunoglobin heavy constant gamma 1 antibody
      • Immunoglobulin gamma 1 antibody
      see all

    Images

    • ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)
      ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)

      A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197767 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

    • ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)
      ELISA - Anti-Mouse IgG antibody [RMG07] (ab197767)

      ELISA of mouse immunoglobulins shows ab197767 reacted to mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197767 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    • Anti-Mouse IgG antibody [RMG07] (ab197767)
      Anti-Mouse IgG antibody [RMG07] (ab197767)

    References (3)

    Publishing research using ab197767? Please let us know so that we can cite the reference in this datasheet.

    ab197767 has been referenced in 3 publications.

    • Otsuka Y  et al. Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice. Biochem Biophys Rep 18:100618 (2019). PubMed: 30805562
    • Sun XY  et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress-induced apoptosis. Mol Med Rep 13:661-8 (2016). IHC-P . PubMed: 26647757
    • Bo H  et al. Upregulation of the expression of Wnt5a promotes the proliferation of pancreatic cancer cells in vitro and in a nude mouse model. Mol Med Rep 13:1163-71 (2016). WB . PubMed: 26648282

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