Recombinant Anti-Mouse IgG antibody [RMG07] (ab197767)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Mouse IgG antibody [RMG07]
See all Mouse IgG primary antibodies -
Description
Goat monoclonal [RMG07] to Mouse IgG -
Host species
Goat -
Specificity
ab197767 reacts to Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG. -
Tested applications
Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Mouse Mouse IgG.
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Positive control
- ELISA: Mouse IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG07 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab197767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 0.1 - 0.5 µg/ml. | |
IP | Use at an assay dependent concentration. | |
ELISA | Use a concentration of 0.05 - 1 µg/ml. | |
IHC-P | Use a concentration of 0.5 - 2 µg/ml. | |
Flow Cyt | Use at an assay dependent concentration. | |
ICC/IF | Use a concentration of 0.5 - 2 µg/ml. |
Target
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Relevance
IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 16017 Mouse
- SwissProt: P01868 Mouse
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Alternative names
- Constant region of heavy chain of IgG1 antibody
- G1m marker antibody
- Gm marker antibody
see all
Images
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A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197767 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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ELISA of mouse immunoglobulins shows ab197767 reacted to mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197767 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Otsuka Y et al. Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice. Biochem Biophys Rep 18:100618 (2019). Read more (PubMed: 30805562) »
- Sun XY et al. Valproate attenuates diabetic nephropathy through inhibition of endoplasmic reticulum stress-induced apoptosis. Mol Med Rep 13:661-8 (2016). IHC-P . Read more (PubMed: 26647757) »