Product nameMouse IgG ELISA kit - 30 minutes
See all IgG kits
Intra-assay Sample n Mean SD CV% A 1/100 9 10.63µg/ml 5.33 % B 1/100 9 11.56µg/ml 5.33 % C 1/100 9 22.61pg/ml 8.48 % D 1/100 9 28.88µg/ml 10.03 % E 1/100 9 66.82µg/ml 8.39 % F 1/100 9 75.92µg/ml 9.9 % G 1/100 9 102.47µg/ml 10.38 % Inter-assay Sample n Mean SD CV% H 1/250 30 3.45 % H 1/500 30 2.99 % H 1/1000 30 4.97 %
Sample typeCell culture supernatant, Ascites Fluid
Assay typeSandwich (quantitative)
Range20 ng/ml - 1900 ng/ml
Assay durationOne step assay
The Mouse IgG quantification Kit (ab273150) provides a rapid and easy method (one step ELISA) to ) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
The kit includes ready-to-use reagents necessary to analyze up to 90 samples in 30 min. Buffer solutions are color coded in order to simplify pipetting steps.
A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10 x 96 tests Detection antibody 1 x 12ml 1 x 120ml Mouse IgG standard 0.0 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 0.02 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 0.1 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 0.2 µg/ml 6 x 0.3ml 6 x 1ml Mouse IgG standard 0.75 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 1.9 µg/ml 1 x 0.3ml 1 x 1ml Pre-coated 8 microwells strips 1 x 96 tests 10 x 96 tests Sample Diluent 1 x 30ml 1 x 500ml Stop Solution 1 x 12ml 1 x 60ml TMB Substrate 1 x 12ml 1 x 60ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
Our Abpromise guarantee covers the use of ab273150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab273150 has not yet been referenced specifically in any publications.