Overview

  • Product name

    Mouse IgG ELISA Kit
    See all IgG kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall 3 3.3%
    Inter-assay
    Sample n Mean SD CV%
    overall 4 2.3%
  • Sample type

    Serum, Cell culture media, Mouse IgG
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 89 % - %
    Cell culture media 85 % - %
    Goat Serum 96 % - %

  • Assay time

    3h 15m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Goat, Cow
  • Product overview

    ab151276, an IgG mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IgG in mouse serum, plasma and supernatant from cell cultures.

     

    This assay employs a mouse IgG specific antibody coated onto plate strips. Standards and samples are pipetted into the wells and IgG present in the sample is bound to the wells by the immobilized antibody.  The wells are washed and an HRP-conjugated anti-mouse IgG detector antibody is added. After washing away unbound detector antibody, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG bound.  The developing blue color is measured at 600 nm.  Optionally the reaction can be stopped with the Stop Solution which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Notes

    There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue.  In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3.  The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure.  The resulting tetramer creates two identical halves which together form a Y like structure.  While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membrane.  IgG is involved in response to a foreign antigen.  The presence of IgG usually signifies a mature antibody response.  IgG has a molecular weight of about 150 kDa, it can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity.  Typically mouse serum and plasma samples contain about 7 to 10 mg/ml of IgG.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Blocking Buffer 1 x 6ml
    10X GAM (H+L)-HRP Detector Antibody 1 x 1ml
    20X Buffer 1 x 20ml
    HRP Development Solution 1 x 6ml
    IgG Mouse Microplate 1 unit
    Normal Mouse IgG Standard (1 ug) 1 x 1µg
    Stop Solution 1 x 12ml
  • Research areas

  • Cellular localization

    Secreted
  • Alternative names

    • Ig gamma 1 chain C region
    • Ig gamma 2 chain C region
    • Ig gamma 3 chain C region
    • Ig gamma 4 chain C region
    • IgG
    • IGHG1
    • IGHG2
    • IGHG3
    • IGHG4
    • Immunoglobin heavy constant gamma 1
    • Immunoglobulin G
    see all

Applications

Our Abpromise guarantee covers the use of ab151276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • A dilution series of standard mouse immunoglobulin G (IgG) in the working range of the assay.
  • Top image: Example dilution series of normal mouse serum (NMS) in the working range of the assay. Bottom image: Example dilution series of a mouse hybridoma cell culture media (TCM) in the working range of the assay.

Protocols

References

This product has been referenced in:

  • Smith B  et al. Generation of two high affinity anti-mouse FcRn antibodies: Inhibition of IgG recycling in wild type mice and effect in a mouse model of immune thrombocytopenia. Int Immunopharmacol 66:362-365 (2019). Read more (PubMed: 30529500) »
  • Ma J  et al. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation. EBioMedicine 22:143-154 (2017). Read more (PubMed: 28711483) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

The antibodies used in this kit recognize mouse IgG (H+L) and do not differentiate between IgG1 and IgG2b so this kit should work and give similar results regardless of whether you have IgG1 or IgG2b in your samples. This kit is designed for quantitatively measuring total mouse IgG, it can also be used for purified IgG.

If you need to quantify an isotype specific mouse IgG, you can also check other Abcam products (IgG1-ab133045); (IgG2a-ab133046); (IgG2b-ab136941); (IgG2c-ab157720) ; (IgG3-ab157721) for a more specific result.

Read More
Abreviews
ELISA kit anti-IgG works on BRAIN sample preparation (after protein extraction).
1% of triton does not interfere with the results.
1X incubation buffer is used for dilution of brain sample.
The brain sample should be diluted for a final range concentration of 200 to 1400 µg/mL of total protein (no tested out of this range). Optimal dilution is 400 µg/mL.

Ms. Marine Poittevin

Verified customer

Submitted Dec 05 2014

Answer

This is an untested sample but it should be an appropriate application with the mouse IgG ELISA kit. The lab's concern is that they have not tested the cross reaction with rabbit IgG. We know rat serum will be detected by this ELISA kit but the signal is relatively much weak. We suggest to run a negative control, i.e. no antibody but vehicle treated sample, to calibrate background issue. We ask to be careful when preparing sample to avoid contamination from rabbit blood.

Read More

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