Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Goat monoclonal [RMG05] to mouse IgG fab
- Suitable for: WB, ELISA, IHC-P, ICC, Flow Cyt, IP
- Reacts with: Mouse
Product nameAnti-mouse IgG fab antibody [RMG05]
DescriptionGoat monoclonal [RMG05] to mouse IgG fab
Specificityab197774 reacts to the Fab region of Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to mouse IgG fab.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab197774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml.|
|ELISA||Use a concentration of 0.05 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
|ICC||Use a concentration of 0.5 - 2 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
ELISA of mouse immunoglobulins shows ab197774 reacted to the Fab region of mouse IgG1, IgG2a, and IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ab197774 has not yet been referenced specifically in any publications.