Recombinant

Recombinant Anti-mouse IgG fab antibody [RMG05] (ab197774)

Overview

  • Product name

    Anti-mouse IgG fab antibody [RMG05]
  • Description

    Goat monoclonal [RMG05] to mouse IgG fab
  • Host species

    Goat
  • Specificity

    ab197774 reacts to the Fab region of Mouse IgG, including IgG1, IgG2a, IgG2b, and IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to mouse IgG fab.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RMG05
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab197774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml.
ELISA Use a concentration of 0.05 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.
ICC Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Images

  • A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • ELISA of mouse immunoglobulins shows ab197774 reacted to the Fab region of mouse IgG1, IgG2a, and IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197774 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab197774 has not yet been referenced specifically in any publications.

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