Recombinant

Recombinant Anti-mouse IgG Fc antibody [RMG06] (ab197780)

Overview

  • Product name

    Anti-mouse IgG Fc antibody [RMG06]
  • Description

    Goat monoclonal [RMG06] to mouse IgG Fc
  • Host species

    Goat
  • Specificity

    ab197780 reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.
  • Tested applications

    Suitable for: IHC-P, WB, ELISA, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse mouse IgG Fc.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RMG06
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab197780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 2 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml.
ELISA Use a concentration of 0.05 - 1 µg/ml.
ICC/IF Use a concentration of 0.5 - 2 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Images

  • A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of ab197780 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.

  • ELISA of mouse immunoglobulins shows ab197780 reacted to the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab197780 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab197780 has not yet been referenced specifically in any publications.

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