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  1. Link

    mouse-igg-isotype-control-ab37355.pdf

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Isotype/Loading Controls Isotype Controls Mouse
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Mouse IgG - Isotype Control (ab37355)

  • Datasheet
  • SDS
Q&A (6)References (54)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

ELISA - Mouse IgG - Isotype Control (ab37355)

    You may also be interested in

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    Overview

    • Product name

      Mouse IgG - Isotype Control
      See all Mouse isotype controls
    • Specificity

      This antibody has been selected as an isotype control as it has no known specificity.

    • Tested applications

      Suitable for: ELISA, Flow Cyt, IHCmore details
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 8.20
      Constituent: 100% Borate buffered saline
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      Purified from normal mouse serum.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Isotype/Loading Controls
      • Isotype Controls
      • Mouse
    • Alternative names

      • Mouse Isotype Control

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab37355 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

     

    IHC
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

     

    IHC
    Use at an assay dependent concentration.

    Images

    • ELISA - Mouse IgG - Isotype Control (ab37355)
      ELISA - Mouse IgG - Isotype Control (ab37355)
      ELISA plate was coated with serially diluted Mouse IgG-UNLB (ab37355). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT, Goat Anti-Mouse IgA-BIOT, and Goat Anti-Mouse IgM, Human ads-BIOT followed by Streptavidin-HRP and quantified.

       

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (54)

    Publishing research using ab37355? Please let us know so that we can cite the reference in this datasheet.

    ab37355 has been referenced in 54 publications.

    • Mirra S  et al. ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity. Cancers (Basel) 13:N/A (2021). PubMed: 33807672
    • Fassler M  et al. Engagement of TREM2 by a novel monoclonal antibody induces activation of microglia and improves cognitive function in Alzheimer's disease models. J Neuroinflammation 18:19 (2021). PubMed: 33422057
    • Small AG  et al. Vitamin D upregulates the macrophage complement receptor immunoglobulin in innate immunity to microbial pathogens. Commun Biol 4:401 (2021). PubMed: 33767430
    • Hoskins EC  et al. Pre-implantation exogenous progesterone and pregnancy in sheep: I. polyamines, nutrient transport, and progestamedins. J Anim Sci Biotechnol 12:39 (2021). PubMed: 33663606
    • Yu L  et al. "Metalloestrogenic" effects of cadmium downstream of G protein-coupled estrogen receptor and mitogen-activated protein kinase pathways in human uterine fibroid cells. Arch Toxicol 95:1995-2006 (2021). PubMed: 33818655
    View all Publications for this product

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-6 of 6 Q&A

    Question

    Please compare with your product ab37355 with our requirements and determine whether these are comparable – and if not, itemize the differences.

    I suspect we might require a custom part number if you can provide them.

    Also verify that the source serum has a U.S. origin. We are looking to get away from foreign source as the supply can be inconsistent.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 19 2012

    Answer

    While previous lots have come from other countries, our most recent lot of ab37355 is from aUS source.I suspect we will continue to try and obtain the serum from this US supplier; however, I would not be able to guarantee this. There are many factors involved when we have to alternate suppliers, such asavailability, cost, quality, etc.If you have aspecific request, please indicate it on the P/O and we will try to accommodate youor let you know an estimated availability date.

    I hope that this is helpful. Please let me know if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 19 2012

    Question

    Thank you for your assistance.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    I'm happy to help! I'll forward information regarding source of the serum ASAP.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    Question


    Attached is Quidel’s internal specification for the Mouse IgG we currently purchase.



    Please compare with your product ab37355 with our requirements and determine whether these are comparable – and if not, itemize the differences.

    I suspect we might require a custom part number if you can provide them.



    Also verify that the source serum has a U.S. origin. We are looking to get away from foreign source as the supply can be inconsistent.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2012

    Answer

    Thanks for sending the attachment. Below I have compared the provided data to the data for ab37335:

    1. Appearence of ab37355 - clear

    2. Concentrationof ab37355 - 5mg/ml

    3. Purification of ab37355- Protein A or G purified

    4. Buffer of ab37355: 100mM Borate buffered solution, pH8.2

    5. Preservative of ab37355: NONE

    6. Storage conditions of ab37355 - Store at -20 degrees C for long term. 4 deg C storage OK for 1-2 weeks.No not recommendingto dilute antibody when aliquoting for storage.

    7. Shipping of ab37355 - Stored frozen, shipped on wet ice

    8. Certificate of ab37355 - A Certificate of Compliance is available on request

    Please note I am still waiting for a reply from the lab regarding source of the serum.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2012

    Question

    Hello,

    Yes I would be interested by your offer.
    I already ask for a quote (see below) to orders@abcam.com
    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 20 2012

    Answer

    Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab37361 and ab37355 in ChIP.

    Thank you also for requesting the proforma invoice. We do not have smaller sizes of these antibody controls. The proforma my colleague sent you, is the smallest size we have.

    Please find here the discount codes:
    xxxx


    I am very pleased to hear you would like to accept our offer and test ab37361 and ab37355 in ChIP.Each of thesecodes will give you: 1 free antibody order before the expiration date. To redeem this offer, please submit an Abreview forChIP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 20 2012

    Question

    Yes, I would like to test them in ChIP experiment.
    Would it be possible to have a quote for one IgG mouse and one IgG rat?

    Thank you in advance,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 18 2012

    Answer

    Thank you very much for your interest in our mouse and rat IgG. The offer consists actually in a 100% discount on a second purchase : please see here for the details:

    As to our knowledge,the mouse IgG fraction ab37355 and the rat IgG fraction ab37361 havenot yetbeen tested in ChIP. Therefore, I can offer a discount off a future purchase if you buy ab37361 and/or ab37355 now, test it inChIP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of:1 freeprimary antibodyfor each antibody tested- so2 free antibodies ifyou test both, ab37361 and ab37355.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab37361 and/or ab37355 in ChIP. I will then send a discount code. This code must be issued before purchasing ab37361 and ab37355 so please wait for my reply before ordering.

    2. Purchase ab37361 and ab37355 either by phone, fax, or online (www.abcam.com).

    3. Test it in ChIP.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab37361 and ab37355 turns out to be unsuitable for ChIP, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Apr 18 2012

    Question

    I can't find a mouse polyclonal control on your website. Could you please tell me if you do sell one or suggest an alternative for fluorescein conjugation and flowcytometry.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 17 2012

    Answer

    Thank you for contacting us.

    We have the following mouse poyclonal antibody to be used as an isotype control in flow cytometry:

    ab37355 (unconjugated; https://www.abcam.com/Mouse-IgG-ab37355.html)

    ab37356 (FITC conjugated; https://www.abcam.com/Mouse-IgG-FITC-Isotype-Control-ab37356.html)

    In case of the unconjugated isotype control, a conjugation kit can be used to label the antibody: https://www.abcam.com/Easylink
    We do carry several FITC conjugation kits: ab102885, ab102884 and ab102883.

    I hope this information is helpful to you. Please let me know if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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