Mouse IgG1 [B11/6] (Phycoerythrin) - Isotype Control (ab91357)

Overview

  • Product name
    Mouse IgG1 [B11/6] (Phycoerythrin) - Isotype Control
    See all Mouse isotype controls
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    A synthetic hapten

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    B11/6
  • Isotype
    IgG1
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

ab91357 is used as a isotype negative control.

References

This product has been referenced in:
  • Quayle LA  et al. Chemotherapy resistance and stemness in mitotically quiescent human breast cancer cells identified by fluorescent dye retention. Clin Exp Metastasis 35:831-846 (2018). Read more (PubMed: 30377878) »
  • Sun W  et al. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. Biomed Res Int 2017:7529104 (2017). Read more (PubMed: 29201913) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

Thank you for contacting us and your interest in our products.
In answer to your questions:
1. Ab concentration of anti-6X His tag (PE), ab72467
The concentration of this antibody is 69 ug/mL.
2. The F:P ratio of the antibody
The F:P ratio of ab72467 is 1:1
3. A suitable isotype control
I think either of the antibodies you have suggested would be suitable as an isotype control. The first, ab1280, is supplied at a concentration of 23 ug/mL and the F:P ratio is lot dependent. The current lot is a ratio of 0.9. The second antibody, ab91357, is supplied at a concentration of 7.5 ug/mL and the F:P ratio is between 1-2. It is very unlikely that either of these isotype controls will cross react with your Salmonella typhimurium however as we have not tested this ourselves we would be able to guarantee it.
I hope this information has been of help, if you have any further questions, please do not hesitate to ask.

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Answer

Thank you for your inquriy.

I am happy to confirm that we offer a isotype control for ab72467 that is also PE conjugated:

ab91357

https://www.abcam.com/index.html?datasheet=91357 (or use the following: https://www.abcam.com/index.html?datasheet=91357).

ab91357 was not specifically tested to not bind to HIS tags. But we believe it is very unlikely since the immunogen used for this antibody was a vegetal immunogen.

I hope this information is helpful, and wish your customer good luck with their experiments.

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Answer

Thank you for your enquiry. An example of an isotype control you can use for flow with ab33816 is ab91357. I hope this is helpful. Please contact me again if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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