Mouse IgG1 [CT6] - Isotype control (ab81216)

Q&A (3)References (4)

Overview

  • Product name

    Mouse IgG1 [CT6] - Isotype control
    See all Mouse isotype controls

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Guinea pig

  • Immunogen

    This information is considered to be commercially sensitive.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • Purification notes

    Membrane filtration

  • Clonality

    Monoclonal

  • Clone number

    CT6

  • Isotype

    IgG1

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab81216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/20.

References (4)

Publishing research using ab81216? Please let us know so that we can cite the reference in this datasheet.

ab81216 has been referenced in 4 publications.

  • Schehr JL  et al. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1. PLoS One 11:e0159397 (2016). PubMed: 27459545
  • Xiao T  et al. CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites. Cell Rep 12:1704-14 (2015). PubMed: 26321640
  • Scheper RJ  et al. Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance. Int J Cancer 42:389-94 (1988). PubMed: 2458323
  • Tan BT  et al. Production of monoclonal antibodies defining guinea pig T-cell surface markers and a strain 13 Ia-like antigen: the value of immunohistological screening. Hybridoma 4:115-24 (1985). Guinea pig . PubMed: 3891587

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

I purchased Anti-Negative control for mouse IgG1 Ab (ab81216) to assess the effect of other blocking ab on tumor cell growth.
But, what I found was cells treated with IgG1 ab also showed retarded growth similar with blocking ab.
I am wondering if this item is not suitable for functional assay such as cell proliferation due to, for example, sodium azide or something ?

Look forward to hearing from you, soon.

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Answer

Thank you for contacting us.

We have not heard other reports of this, but it is possible the azide in the antibody, which is present at 0.1% in the stock solution, does have an effect on the tumor growth. Confirming this would require an experiment, for instance comparing the effect of medium containing the same amount of azide to the effect of the antibody control.

Alternatively, you could remove the azide by exchanging the buffer of the stock solution. The product ab102776 will accomplish this. Here is a URL for the product:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102776).

Please let me know if you have other questions or concerns.

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Question

Please send us CofC for these two items.

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Answer

Thank you for your reply.

I have attached the certificate of compliance for ab290 and ab81216, with the lot numbers specified.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Please send me CofA for the followings:

Cat# AB290 Lot# GR7657-1

Cat# AB81216 Lot# GR98155-1

Thanks

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Answer

Thank you for your enquiry.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within 6 monthsof purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

The Customer Services and Scientific Support Teams would be very pleased to send a CoC on demand. Could you please let me know if you would stilllike to receive a CoC for these two items?

Please do not hesitate to contact me again if you have further questions.

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