Mouse IgG1 [CT6] - Isotype control (ab81216)

Q&A (3)References (4)

Overview

  • Product name
    Mouse IgG1 [CT6] - Isotype control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Guinea pig
  • Immunogen

    This information is considered to be commercially sensitive.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
  • Concentration information loading...
  • Purification notes
    Membrane filtration
  • Clonality
    Monoclonal
  • Clone number
    CT6
  • Isotype
    IgG1
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab81216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/20.

References

This product has been referenced in:
  • Schehr JL  et al. High Specificity in Circulating Tumor Cell Identification Is Required for Accurate Evaluation of Programmed Death-Ligand 1. PLoS One 11:e0159397 (2016). Read more (PubMed: 27459545) »
  • Xiao T  et al. CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites. Cell Rep 12:1704-14 (2015). Read more (PubMed: 26321640) »
See all 4 Publications for this product

Publishing research using ab81216? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
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1-3 of 3 Q&A

Question

I purchased Anti-Negative control for mouse IgG1 Ab (ab81216) to assess the effect of other blocking ab on tumor cell growth.
But, what I found was cells treated with IgG1 ab also showed retarded growth similar with blocking ab.
I am wondering if this item is not suitable for functional assay such as cell proliferation due to, for example, sodium azide or something ?

Look forward to hearing from you, soon.

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Answer

Thank you for contacting us.

We have not heard other reports of this, but it is possible the azide in the antibody, which is present at 0.1% in the stock solution, does have an effect on the tumor growth. Confirming this would require an experiment, for instance comparing the effect of medium containing the same amount of azide to the effect of the antibody control.

Alternatively, you could remove the azide by exchanging the buffer of the stock solution. The product ab102776 will accomplish this. Here is a URL for the product:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102776).

Please let me know if you have other questions or concerns.

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Question

Please send us CofC for these two items.

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Answer

Thank you for your reply.

I have attached the certificate of compliance for ab290 and ab81216, with the lot numbers specified.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Please send me CofA for the followings:

Cat# AB290 Lot# GR7657-1

Cat# AB81216 Lot# GR98155-1

Thanks

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Answer

Thank you for your enquiry.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within 6 monthsof purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

The Customer Services and Scientific Support Teams would be very pleased to send a CoC on demand. Could you please let me know if you would stilllike to receive a CoC for these two items?

Please do not hesitate to contact me again if you have further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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