Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (ab221848)

Overview

  • Product name

    Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free
    See all Coiled-coil domain-containing protein 113 isotype controls
  • Specificity

    This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cytmore details
  • Immunogen

    Other Immunogen Type. ab221848 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.

  • General notes

    This antibody clone is manufactured by Abcam.

    ab221848 is a PBS only buffer formulated version of ab170190.

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Monoclonal
  • Clone number

    15-6E10A7
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Alternative names

    • CCDC113
    • Coiled-coil domain-containing protein 113 Gene names
    • DKFZp434N1418
    • HSPC065

Applications

Our Abpromise guarantee covers the use of ab221848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 26627833
ICC Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.

Images

  • This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170190).

    Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

    An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

References

This product has been referenced in:

  • Cui Y  et al. The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation. Sci Rep 6:23300 (2016). Flow Cyt . Read more (PubMed: 26996236) »
  • Landa-Solís C  et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank N/A:N/A (2015). Read more (PubMed: 26220398) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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