Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Q&A (11)Specific References (21)

Overview

  • Product name
    Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse. Synthetic hapten

  • General notes

    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab91353 is purified and passed through a 0.22µm filter
  • Isotype control notes
    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
  • Clonality
    Monoclonal
  • Clone number
    B11/6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91353 is a negative isotype control
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Images

  • Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Shafa M  et al. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers. Front Med (Lausanne) 5:69 (2018). Read more (PubMed: 29600249) »
  • Allam O  et al. HIV induces production of IL-18 from intestinal epithelial cells that increases intestinal permeability and microbial translocation. PLoS One 13:e0194185 (2018). Read more (PubMed: 29601578) »

See all 21 Publications for this product

Publishing research using ab91353? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a question

11 Q&A

10
Votes: Highest First

I selected the following antibodies from the ABcam catalog:

Primary:

Anti-CD63 antibody [AD1] (FITC)

ab108949

$337.00



Anti-CD62P antibody [Psel.KO.2.7]

ab54427

$33...

Read More

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please ...

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Dear Tech Support Team,
Kindly advise if the following isotypes controls are the compatible options for the mentioned antibodies:
Isotype control for ab6142 anti-Nestin unconjugated
http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=https...

Read More

Thank you for contacting us.
I can confirm the isotype controls suggested are suitable for the antibodies requested.
The difference in price between ab91353 and ab81197 lies in the volume difference (the volume of ab91353 doubles ab81197’s vo...

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Dear,
I ordered the following antibodies and received them at the end of September:
- Ab54426 (anti-CD62P)
- Ab91353 (Mouse IgG1 – isotype control)
- Ab 18981 (anti-CD62E)
- Ab27478 (rabbit polyclonal IgG)
- Ab2213 (...

Read More

Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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need a C of A for item # AB91353, lot **(Mouse IG1 Isotype Control).

Thank you for contacting Abcam.

Please find attached the Certificate of Compliance for ab91353 (Abcam does not issue Certificate of Analysis).

If there is anything else I can help you with, please let me know.

Dear,
We want to proceed and test ab54426 in IHC-P. You can send me the discount code.
Are these the correct isotype antibodies for the primary antibodies I have chosen:
- Mouse IgG2a, kappa monoclonal [MG2a-53] - isotype control (Ab1841...

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I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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HI,

I BOUGHT

Mouse IgG1 [ICIGG1] - Isotype Control (ab91353)

100 µg at 0.1mg/ml

2µg/1x106 cells


Anti-Cytokeratin 18 antibody [C-04] (ab668)

100 µg at 1mg/ml
...

Read More

Thank you for contacting Abcam.

I am sorry that you are having some issues using ab668 in flow cytometry. The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on host of samples. If we cannot ...

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For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them.
ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> ...

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Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, ...

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Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

Feel free to contact him to double-check, he is the one who gave me the customer ID.

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-7...

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Concerning the quote, the customer number is**********

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just ...

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Phonec all: Customer asked the concentration of mouse IgG1 isotype control.

Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1-10 of 11 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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