Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Q&A (11)References (23)

Overview

  • Product name
    Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse. Synthetic hapten

  • General notes

    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab91353 is purified and passed through a 0.22µm filter
  • Isotype control notes
    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
  • Clonality
    Monoclonal
  • Clone number
    B11/6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

ab91353 is a negative isotype control
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Karanovic et al One. 2016 Aug 25;11(8):e0161706. doi: 10.1371/journal.pone.0161706. eCollection 2016. Fig 9.

    Glomerular nestin expression in experimental groups.

    (A) Negative control (×200).

    (C) SHADR group: Diffuse glomerular nestin expression was detected involving almost all podocytes within glomerulus. After losartan and tempol treatment, either single or in combination, kidneys restored nestin expression similar to controls (SHC group).

    Immunostaining was applied on 5 μm thick paraffin sections. After deparaffinization and rehydration, the sections were treated by microwave for 20 minutes at 400 W in citrate buffer (pH 6.0). After antigen retrieval, samples were incubated for 1 hour at room temperature with primary antibody for nestin (dilution 1:100). Sections were then treated using 3-amino-9-ethylcarbazole (AEC) as substrate, and counterstained with hematoxylin. Negative controls were performed by omitting the first antibody and mouse monoclonal antibodies as isotype control mouse IgG1 (ab91353) antibody was also used.

  • Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)Hirakawa et al PLoS One. 2016 Aug 3;11(8):e0159912. doi: 10.1371/journal.pone.0159912. eCollection 2016. Fig 1.

    Effects of hypoxia on mRNA expression of IGF1R and IGF1, and production of IGF1 in cancer cells.

    Cell surface expression of IGF1R by FACScan analysis. IGF1R expression level of Panc-1, RWP-1, OCUP-AT, and MiaPaCa-2 cells was higher in hypoxia than that in normoxia.

    Cells (2 x 106 cells/mL) were fixed with 2% paraformaldehyde and incubated in PBS with anti IGF1R antibody (ab16890, Abcam) or mouse IgG1- isotype control (ab91353, Abcam) for 30 minutes at 22°C. Cells were subsequently labeled with FITC-conjugated secondary antibody (1:500; ab96879, Abcam) for 30 minutes at 22°C.

    The percentage of positive cells were calculated and compared with isotype-matched control-stained cells.

  • Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab59509 (red line).

    The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC.

    Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Setthawong P  et al. Generation of porcine induced-pluripotent stem cells from Sertoli cells. Theriogenology 127:32-40 (2019). Read more (PubMed: 30639694) »
  • Shafa M  et al. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers. Front Med (Lausanne) 5:69 (2018). Read more (PubMed: 29600249) »
See all 23 Publications for this product

Publishing research using ab91353? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Q&A
Submit a question

1-10 of 11 Q&A

Question

I selected the following antibodies from the ABcam catalog:

Primary:

Anti-CD63 antibody [AD1] (FITC)

ab108949

$337.00



Anti-CD62P antibody [Psel.KO.2.7]

ab54427

$337.00



Anti-CD42d antibody [1C2]

ab95780

$337.00



Secondary:


Goat anti-Mouse IgG H&L (Cy5.5 ®)

ab6947

$185.00



Goat anti-Mouse IgG1 heavy chain (FITC)

ab97239

$165.00



Goat polyclonal to Armenian Hamster (TR)

ab5743

$165.00



Isotype controls:


MOUSE IgG1 antibody [MOPC-21] (FITC)

ab106163

$325.00



Mouse IgG1 [ICIGG1] - Isotype Control

ab91353

$315.00



Armenian Hamster IgG monoclonal [HTK888] - Isotype control

ab18479

$325.00



Two questions:

For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

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Answer

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

I look forward to your reply.

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Question

Dear Tech Support Team,
Kindly advise if the following isotypes controls are the compatible options for the mentioned antibodies:
Isotype control for ab6142 anti-Nestin unconjugated
http://mail.zotal.co.il/exchweb/bin/redir.asp?URL=https://www.abcam.com/Mouse-IgG1-ICIGG1-Isotype-Control-ab91353.html
Isotype control for ab62122 anti-p75 FITC conjugated
https://www.abcam.com/Mouse-IgG2a-FITC-Isotype-control-ab81197.html
Please explain the difference in the pricing of ab91353 and ab81197.
Thanks in advance for your assistance and reply.
Have a nice week.

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Answer

Thank you for contacting us.
I can confirm the isotype controls suggested are suitable for the antibodies requested.
The difference in price between ab91353 and ab81197 lies in the volume difference (the volume of ab91353 doubles ab81197’s volume) and the manufacturing and purification process often make price vary between what seems to be similar products.
I hope this helps. Please do not hesitate to contact us if you require further assistance.

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Question

Dear,
I ordered the following antibodies and received them at the end of September:
- Ab54426 (anti-CD62P)
- Ab91353 (Mouse IgG1 – isotype control)
- Ab 18981 (anti-CD62E)
- Ab27478 (rabbit polyclonal IgG)
- Ab2213 (anti-ICAM1)

I stored them at +4oC till today, but I have noticed today that your storage instructions recommend to aliquot and store most of the antibodies at -20oC or -80oC.
Could this have had an effect on my stainings?
Kind regards,

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Answer

Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4oC for a few weeks should not significantly affect the staining. However, we would recommend to follow the individual storage instructions provided on the individual datasheets as soon as possible after delivery. This will help to ensure that the antibody remains stable and that we can continue to provide our guarantee.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

need a C of A for item # AB91353, lot **(Mouse IG1 Isotype Control).

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Answer

Thank you for contacting Abcam.

Please find attached the Certificate of Compliance for ab91353 (Abcam does not issue Certificate of Analysis).

If there is anything else I can help you with, please let me know.

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Question

Dear,
We want to proceed and test ab54426 in IHC-P. You can send me the discount code.
Are these the correct isotype antibodies for the primary antibodies I have chosen:
- Mouse IgG2a, kappa monoclonal [MG2a-53] - isotype control (Ab18414) for the 1° Ab: Anti-ICAM1 antibody [MEM-111] (Ab2213)
- Rabbit polyclonal IgG (Ab 27478) for the 1° Ab: Rabbit polyclonal to CD62 E (Ab 18981)
- Mouse IgG1 [ICIGG1] - Isotype Control (ab91353) for the 1° Ab: Anti-CD62P antibody (Ab54426)
Kind regards,

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Answer

I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab54426 used in IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

With regards to the isotype controls:
For ab2213, the light chain type is unknown, so I would recommend to use a general IgG2a isotype control rather than a specific IgG kappa chain isotype control. For example the following product:

ab91361 Mouse IgG2a [ICIGG2A] - Isotype Control
https://www.abcam.com/index.html?datasheet=91361 https://www.abcam.com/index.html?datasheet=91361.

However, please note this is not tested in IHC-P. Unfortunately, I am sorry we do not have any suitable isotype controls tested in IHC-P in this case.

ab27478 is a suitable isotype control for ab18981
ab91353 is a suitable isotype control for ab54426

Any feedback that you can provide regarding ab54426 will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

HI,

I BOUGHT

Mouse IgG1 [ICIGG1] - Isotype Control (ab91353)

100 µg at 0.1mg/ml

2µg/1x106 cells


Anti-Cytokeratin 18 antibody [C-04] (ab668)

100 µg at 1mg/ml

1µg/1x106 cells

WHEN I USED THEM FOR FLOW I SAW THAT NEGATIVE WAS STRONGER AND HIGHER THAN POSITIVE.

DO I GET IT RIGHT THAT 1µg CYT18 - IS 1 MKL AND THE SAME FOR CONTROL ABS IS 10 MKL?

PLEASE HELP ME TO UNDERSTAND!!!

ALSO I USED PARAFORMALDEHIDE INSTEAD OF METHANOL AS IT SASY THAT STAINING WAS THE SAME.

AS I TRIED THIS FOR CELLS THAT WERE FIRST STAINED FOR SURFACE MARKERS I WAS AFRAID OF LOOSING SIGNAL AFTER METHANOL.

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Answer

Thank you for contacting Abcam.

I am sorry that you are having some issues using ab668 in flow cytometry. The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on host of samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to aid you further, would you be able to send me a copy of the entire protocol that you use, including whether you permeabilized the cells or not and if you can send an example of the results you are getting, that would also be helpful.

Looking forward to your reply and helping you resolve this issue.

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Question

For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them.
ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG
In the list I was asking for isotype controls, but still the positive controls would be interesting to know, too.
All the best,

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Answer

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

Isotype controls:



can be searched at this site: https://www.abcam.com/index.html?c=718

ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032



Positive control:



ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

ab66219 -- Oprl1 - Brain (cortex),

ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

Feel free to contact him to double-check, he is the one who gave me the customer ID.

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Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

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Question

Concerning the quote, the customer number is**********

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Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

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Question

Phonec all: Customer asked the concentration of mouse IgG1 isotype control.

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Answer

Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 11 Q&A

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