Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control (ab18448)

Q&A (5)Specific References (4)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes


    The ab18448, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The immunoglobulin was purified by affinity chromatography.
  • Isotype control notes
    The ab18448, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality
    Monoclonal
  • Clone number
    MG1-45
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

References

This product has been referenced in:
  • Yuan SM  et al. Over-expression of PPAR-?2 gene enhances the adipogenic differentiation of hemangioma-derived mesenchymal stem cellsin vitroandin vivo. Oncotarget 8:115817-115828 (2017). Read more (PubMed: 29383203) »
  • Feng H  et al. NLRX1 promotes immediate IRF1-directed antiviral responses by limiting dsRNA-activated translational inhibition mediated by PKR. Nat Immunol 18:1299-1309 (2017). Read more (PubMed: 28967880) »

See all 4 Publications for this product

Publishing research using ab18448? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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5 Q&A

10
Votes: Highest First

Bonjour,



Voici le document rempli avec des résultats de FACS, les photos IF et le WB.

L’anticorps abcam marche en IF et WB, par contre l’isotype reconnait une bande de la même taille que K10 en WB&h...

Read More

Mercipour votre réponse ainsi que pour le questionnaire rempli et détaillé.

Il m'est toujours impossible de trouver une explication définitive à votre problème.
Est-ce que l'anticorps anti-K10 est ...

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Problèmes en FACS et IFavec ab9025 (primaire) et ab18448 (controle isotype).
Anticorps couplés avec kit invitrogen "zenon"

controles négatifs : HEK

marquage positif obtenus dans tous les cas de figures.

En plus de vos résultats obtenus en Western Blot, vous serait-il possible de remplir et nous retourner le questionnaire ci-joint?

Les informations sur le protocole utilisé nous sera utile pour tenter de comprendre le problè...

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Problèmes en FACS et IFavec ab9025 (primaire) et ab18448 (controle isotype).
Anticorps couplés
controles négatifs : HEK

marquage positif obtenus dans tous les cas de figures.

Merci de nous avoir contactés.

Je vous envoie comme convenu un email auquel vous pourrez répondre et joindre vos résultats en Western Blot avec les anticorps ab9025 et ab18448.
Votre réponse me sera alors automa...

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Phonec all: Customer asked the concentration of mouse IgG1 isotype control.

Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Phonec all: Customer asked the concentration of mouse IgG1 isotype control.

Thank you for contacting us. I can confirm that product ab91353 can also be used as normal isotype control with human cells or in applications as stated on the datasheet. I have also sent an email to my colleague in lab about your requir...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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