Mouse IgG1, kappa monoclonal [MG1-45] - Isotype Control - BSA/Azide free (ab18447)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MG1-45] - Isotype Control - BSA/Azide free
    See all Mouse isotype controls
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, Flow Cyt, WB, IPmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes

    The ab18447, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab18447 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.
  • Isotype control notes
    The ab18447, MG1-45 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality
    Monoclonal
  • Clone number
    MG1-45
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 18006877
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. Use at an assay dependent dilution for surface or intracellular staining.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

References

This product has been referenced in:
  • Ferreira SA  et al. Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence. Biomaterials 176:13-23 (2018). Read more (PubMed: 29852376) »
  • Levet S  et al. An ancestral retroviral protein identified as a therapeutic target in type-1 diabetes. JCI Insight 2:N/A (2017). IHC-P . Read more (PubMed: 28878130) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 11 Q&A

Answer

I can confirm that ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control would be a suitable isotype control for use with ab46678 CD14 antibody [MEM-18]. It has been tested and therefore covered by the guarantee in functional studies.

ab18447
https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

I hope this will be helpful. if you have any further questions, please do not hesitate to contact me.

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Answer

Tom is currently away from the office. Thus, I will be helping you. My name is Sybille.

As for ab18447, it is a protein G purified antibody that comes in PBS without any preservatives. As per datasheet, ab18447 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.

We have the human IgG1 protein (ab90283) that was purified human myeloma plasma and comes in 20mM PBS, 150mM Sodium chloride, pH 7.4 with 0.05% sodium azide. The nature of the light chain is kappa.

There would be no human raised monoclonal antibodies as antibodies are not raised in humans. Human antibodies are only purified from human blood/plasma.

Were you looking for a monoclonal antibody that is specific to a certain target?

Please let me know if I can help you in any other way.

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Answer

I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Abcam also carries other products essential for Flow Cytometry such as isotype controls which confirm that the primary antibody binding is specific and not results of non-specific Fc receptor binding or other protein interactions. The isotype control should match the species, isotype, clonality and conjugation of the primary antibody. In this case the isotype control ab56670 is: Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414) and for ab60719: Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control (ab18447). More information about these isotype controls may be found at the following locations: Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414): https://www.abcam.com/mouse-igg2a-kappa-monoclonal-mg2a-53-isotype-control-ab18414.html Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control (ab18447): https://www.abcam.com/mouse-igg1-kappa-monoclonal-mg1-45-isotype-control-ab18447.html If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit. Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html 7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

The concentration of this lot is GR91861-1 is 0.85mg/ml. An appropriate isotype control would be ab91353, Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. This product is shipped with a concentration of 1mg/ml. https://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html We recommend use of a pre-absorbed secondary antibody in Flow Cytometry. We have a number of pre-absorbed anti-Mouse IgG1 antibodies which are conjugated to fluorescent molecules for use in Flow. Choice of the appropriate marker will depend upon your experimental set up and flow cytometer. I have complied a short list for you below. Secondary Antibodies: AB98692 (FITC conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-FITC-pre-adsorbed-ab98692.html AB136127 (Cy5 conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-gamma-chain-Cy5--pre-adsorbed-ab136127.html AB130785 (AP/Cy7 conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-AllophycocyaninCy7-pre-adsorbed-ab130785.html AB130786 (AP conjugated): https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG1-heavy-chain-Allophycocyanin-pre-adsorbed-ab130786.html

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Answer

Thank you for contacting us.

We do not currently have a pan- mouse IgG isotype control suitable for in vivo use on mouse. We do have isotype controls for each IgG subtype which have beenLEAF Purified. This is a low endotoxin azide free formulation containing no preservative and is 0.2 µm filter sterilized the endotoxin level for these products is <0.01 ng/µg protein. Further these have beenscreened on a variety of resting, activated, live, and fixed mouse tissues.

ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype controlhttps://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html

ab18414Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype controlhttps://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18414.html

ab18457Mouse IgG2b, kappa monoclonal [MPC-11] - isotype control. BSA/Azide freehttps://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MPC-11-isotype-control-BSA-Azide-free-ab18457.html

ab18392Mouse IgG3, kappa monoclonal [MG3-35] - Azide freehttps://www.abcam.com/Mouse-IgG3-kappa-monoclonal-MG3-35-Azide-free-ab18392.html

Should you be interested in these products at the volumes which you have specified these would be considered bulk purchases and may qualify for special pricing. Please contact sales@abcam.com for more information about this.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank youfor your inquiry.

I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

Isotype control are not included and have to be purchased separately.

ab41616 (Anti-Dengue Virus NS1 glycoprotein antibody [DN3]):

https://www.abcam.com/index.html?datasheet=41616 (or use the following: https://www.abcam.com/index.html?datasheet=41616).

Heaton NS et al. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc Natl Acad Sci U S A 107:17345-50 (2010). ICC/IF. PubMed: 20855599

Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

ab41623 (Anti-Dengue Virus NS1 glycoprotein antibody [DN2]):

https://www.abcam.com/index.html?datasheet=41623 (or use the following: https://www.abcam.com/index.html?datasheet=41623).

Smith KM et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J 8:289 (2011). IF. PubMed: 21658241

Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

ab41490 (Anti-Dengue Virus NS1 glycoprotein antibody [DN1]):

https://www.abcam.com/index.html?datasheet=41490 (or use the following: https://www.abcam.com/index.html?datasheet=41490).

Currently we do not know of any references using this antibody.

Isotype control: ab18449 (Mouse IgG2a, kappa [MOPC-173] (low endotoxin) - isotype control)
https://www.abcam.com/index.html?datasheet=18449 (or use the following: https://www.abcam.com/index.html?datasheet=18449).

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

We do not sell directly to Pakistan. Please contact our trusted distributor for pricing information:

Friends Scientific Corporation
3-B Ali Continental,
1-Mozang Road
Lahore
54000
Pakistan

Tel 4237236176 Fax 4237321500 Email mailto:friends1@wol.net.pk

I hope this information is helpful and wish you good luck with your experiments.

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Question
Answer

Here are the MSDS forms you requested. Please contact us again if you need anything further.

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Answer

Thank you for your inquiry and I apologize for the delay in this reply. Yes, ab18447 is a suitable isotype control for ab17199. https://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting us. Could you please specify which product you would be interested in buying? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us. I can confirm that product ab91353 can also be used as normal isotype control with human cells or in applications as stated on the datasheet. I have also sent an email to my colleague in lab about your requirements and now waiting a reply from them. I will get back to you soon. Should you have any other question please do not hesitate to contact me.

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1-10 of 11 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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