Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control (ab18435)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control
    See all Mouse isotype controls
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Conjugation notes
    The solution is free of unconjugated FITC.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    KLH

  • General notes
    Isotype control has been screened on a variety of resting, activated, live, and fixed rat and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    MOPC-21
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution. (surface or intracellular staining)

References

ab18435 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Q&A

Answer

Thank you for contacting us.

Unfortunately we have not determined the exact concentration for this product. Before conjugation with FITC this product has a 1mg/ml concentration. When using isotype controls in Flow Cytometry keep in mind that it is recommended that concentrations are really antibody amount in excess. Therefore we would recommend using ab18435 Mouse IgG1 Isotype control at a concentration matching or exceeding 1mg/ml.

Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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Answer

Thank you very much for your inquiry.

Yes, you are absolutely correct. I can confirm, that the non- conjugated equivalent of the ab86886 is the ab86885.

When using an unconjugated antibody I can however also recommend to use an unconjugated isotype control. We forexample the ab91353as an mouse IgG1 unconjugatedisotype control.

I hopethis information is helpful. Please do not hesitate to contact us again should you haveany further question.

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Answer

Thank you very much for your inquiry.

For the ab51387 I recommend to use an isotype control which is of the same isotype and conjugation. I can therefore not recommend to use the ab18435 for this antibody as a control.

We do currently not have the optimal control for ab51387, which is a mouse IgG1 conjugated to PE and TR in our catalogue. I have inquired with my colleagues whether we can add this for you. I will contact you again, as soon as I have received an answer.

Thank you for your patience. Please do not hesitate to contact us also meanwhile, should you have any questions or concerns.

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Answer



The anti-CD4 antibody [RPA-T4] (FITC), ab86886, is a Mouse IgG1. The best isotype control for ab86886 would be a Mouse IgG1 FITC conjugated such as ab18435.

ab37356 would be a good isotype control for a Mouse polyclonal IgG FITC conjugated.

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Answer

Thank you for contacting us. Mouse IgG1, kappa monoclonal [MOPC-21] (FITC) - Isotype control, ab18435, is produced in ascites. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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