Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control
    See all Mouse isotype controls
  • Specificity
    MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live and fixed rat and human tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WBmore details
  • Immunogen

    Other Immunogen Type corresponding to Mouse. There is no immunogen for this product because it was not generated through a hybridoma. It was generated by the myeloma of a mouse that was fed mineral oil.

  • General notes

    ab18443 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Isotype control notes
    ab18433 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    MOPC-21
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

This product has been referenced in:
  • Perry DL  et al. Ebola Virus Localization in the Macaque Reproductive Tract during Acute Ebola Virus Disease. Am J Pathol 188:550-558 (2018). IHC-P . Read more (PubMed: 29429544) »
  • Werner TA  et al. CXCR4/CXCR7/CXCL12-Axis in Follicular Thyroid Carcinoma. J Cancer 9:929-940 (2018). WB, IHC-P . Read more (PubMed: 29581772) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Thank you for contacting us.

If you provide me with the institution that placed the order, I will be able to tell you the exact lot number you purchased. However, roughly 4 months ago we were selling lot GR54839-4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. According to my literature 99% of mouse IgG immunoglobulin molecules have a kappa light chain. This is reflected in the number of IgG, kappa isotype controls in our catalogue. Therefore given that your three mouse antibodies are IgG1; with the light chain unspecified in two of them and one specified as kappa I think that it is safe to assume that you can use a single isotype control relating for all three antibodies relating to heavy chain IgG1 and kappa light chain. For example; ab18443 Mouse IgG1, kappa monoclonal (MOPC-21) - isotype control or ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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