Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype Control (Low endotoxin, Azide Free) (ab18437)

Overview

  • Product name
    Mouse IgG1, kappa monoclonal [MOPC-21] - Isotype Control (Low endotoxin, Azide Free)
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, IHC-Frmore details
  • General notes
    The ab18437, MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.


    The ab18437, MOPC-21 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
    0.2µm filter sterilized. Endotoxin level is <0.01 ng/µg protein.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab18437 is low endotoxin and azide free. The antibody was purified by affinity chromatography.
  • Isotype control notes
    The ab18437, MOPC-21 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality
    Monoclonal
  • Clone number
    MOPC-21
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References

ab18437 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

The concentration is determined as follows:

When a new lot is produced the lab takes an aliquot and measure the OD of that aliquot multiple times and a mean is calculated. Then a second aliquot is measured independently, also several times and the mean is calculated. Then these two means are compared, if they differ by more than 5% the lot is not approved until further investigation reveals why the difference is more than 5%, and then corrected.

However, they do not record standard deviation. They only record the mean between the two aliquots (even when it is implied by the method that the CV is less than 5%).

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Question
Answer

Thank you for your enquiry. The antibody concentration is 1 mg/ml. Please do not hesitate to contact us if you have additional questions.

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Question

BATCH NUMBER 181315 ORDER NUMBER 141240 DESCRIPTION OF THE PROBLEM Non-specific staining We purchased this isotype control antibody for use in immunohistochemistry (paraffin) as a control for anti-human Foxp3 (ab20034). While the Foxp3 antibody gives an expected clean positive signal without background on human tonsil, your isotype control gives a very high non-specific background staining at a matched concentration. We are using the anti-Foxp3 at a lower concentration than you recommend as it stains strongly, thus we are working within the concentration range that this isotype control should work at. We also have CD4 staining working which is also a mouse IgG1 antibody, and this also gives a cleaner background than your isotype control. Thus, the staining protocol works but there is specific problem with this isotype control. If it were a general blocking issue then then all the antibodies would give similar backgrounds at the same concentration. SAMPLE human tonsil PRIMARY ANTIBODY Abcam, anti-human Foxp3, mouse IgG1, 1/100 (recomended 1/40), o/n 4oc or 1hr RT. Wash:3x10 minutes in PBS. DETECTION METHOD R & D Kit (both DAB and AEC) POSITIVE AND NEGATIVE CONTROLS USED secondary alone - clean signal, no background your isotype - high background Substrate alone - no background streptavidin and substrate (no secondary) - no background ANTIBODY STORAGE CONDITIONS 4 degrees (as it arrived) FIXATION OF SAMPLE paraformaldehyde, paraffin sections ANTIGEN RETRIEVAL Heat-microwave, Tris/EDTA pH9. This works, we get a signal for both Fp3 and CD4. BLOCKING CONDITIONS R & D Kit (both DAB and AEC) SECONDARY ANTIBODY R & D Kit (both DAB and AEC) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5-6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

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Answer

Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab18437. I think the problem might be due to the fact that ab18437 is pure IgG1 kappa, while ab20034 is made of a mixture of kappa and lambda. Therefore they are not truly comparable and a mixture of lambda and kappa isotype would be better (for example abab27479 Mouse IgG1 [NCM1])and the concentration of IgG1 kappa in the isotype would be matching that of ab20034. I hope this suggestion will help you, please do not hesitate to contact me if you need further assistance,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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