Mouse IgG1, Kappa Monoclonal (NCG01) - Isotype Control - BSA and Azide Free (ab81032)

Overview

  • Product name
    Mouse IgG1, Kappa Monoclonal (NCG01) - Isotype Control - BSA and Azide Free
    See all Mouse isotype controls
  • Specificity
    This mouse monoclonal IgG1 was produced against a synthetic hapten, which is normally not present in humans or animals. Therefore, this mouse IgG1 does not react with any antigen of human or animal cells.
  • Tested applications
    Suitable for: WB, IP, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Does not react with any antigen of human or animal cells.
  • Immunogen

    Synthetic hapten.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    Purified from ascites.
  • Clonality
    Monoclonal
  • Clone number
    NCG01
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab81032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.

References

This product has been referenced in:
  • Guo J  et al. Interleukin-1ß induces intercellular adhesion molecule-1 expression, thus enhancing the adhesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. Int J Mol Med 41:1976-1982 (2018). Read more (PubMed: 29393395) »
  • Tougan T  et al. Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. Sci Rep 8:5052 (2018). Read more (PubMed: 29567995) »
See all 13 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Answer

Thank you for contacting us.

We have sourced this antibody from external collaborator laboratory. Unfortunately they have not released this information to us so I am sorry we are unable to help in this case.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

Isotype controls:



can be searched at this site: https://www.abcam.com/index.html?c=718

ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032



Positive control:



ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

ab66219 -- Oprl1 - Brain (cortex),

ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Good day
I have a question about ab81032 product - Negative control IgG1.We had bought this Negative control IgG1 post-sale.
This IgG1 can’t attache to anywhere and dosen’t give any signals.
We had used MABA4 in normal reaction - this antibody used in publication: Mapping epitope specificities of monoclonal antibodies to thyroid peroxidase using recombinant antigen preparations.Ewins DL, Barnett PS, Tomlinson RW, McGregor AM, Banga JP.Autoimmunity. 1992;11(3):141-9.
This antibody we receive in Mouse. Isotype this antibody it's IgG1.
I want check signal MABA4 with MCF7 cell line, becouse we'll provide good reaction with MCF7 and MAB4. Positive reaction we have with CHO-TPO(mutants CHO with TPO), and IgG1 it'll be negative control for reaction with MCF7 cell line, and we wouldn't have any signals there, becouse negative control wouldn't give signals anywhere. But we have signals in ICC and Western blot. Probably it was not specific signal but it was in Western blot and ICC.
In our research we have positive reaction with IgG1 in immunocytochemistry and Western blot.
We use IgG1 –in ICC- in three solutions: 0.2mikrograms/ml, 0.1 mikrograms/ml, 0.05mikrograms/ml and secondary antibody (Jackson Immuno- dyLight 549 Goat anti Mouse) in two solutions 1:800 and 1:1000, and in this two cases we have positive reaction.
We had blocked reaction (ICC) 1hr, RT, with goat serum + bovine serum - 5% and in this serum we solution IgG1 and antibody.
In blot we use 1 mikrogram/ml IgG1.
I send You our photos with that reactions:
1- there is described IgG1 0.2 mikrograms/ml and 0.05 mikrograms/ml (with MCF7 cell line) – secondary antibody there is 1:800 solutions,
2- there is IgG1 0.2/ml, 0.1/ml, 0.05/ml and secondary antibody 1:1000 (with MCF7 cell line).
Controls for secondary antibody are good.
3-Western Blot – there is Western blot with described IgG1
Could You give me advice about this IgG1. What we can do?
Best regards,

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Answer

Thank you for contacting us.

I am sorry to hear you are having unsatisfactory results with one of our products.

Ab81032 is an isotype control, therefore it doesn’t recognise any target of Human or animal cells. In your email you mention it doesn’t attach to anywhere as the reason for the complaint. As this antibody was raised against a synthetic hapten, not present in humans or animal cells, no signal is expected from it.

Is the primary antibody being used together with the Isotype control? What optimisation steps have been done?

I would appreciate if you could please provide the order number and the lot number in order to check the lot specific records.

Our policy is that we will offer pre- and post-sale technical support for products sold via unauthorized distributors. In case of problems with our antibodies we will offer suggestions to the researcher's protocol but we will not offer our guarantee as we cannot control the shipping and storage conditions managed by the unauthorized distributor.

Thank you for your cooperation. I look forward to receiving your reply.

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Answer

Thank you for contacting us and sending us the protocol and the images. I am sorry to hear your customer is having problems with one of our products.

Our policy is that we will offer pre- and post-sale technical support for products sold via unauthorized distributors. In case of problems with our antibodies we will offer suggestions to the researcher's protocol but we will not offer our guarantee as we cannot control the shipping and storage conditions managed by the unauthorized distributor.

In order to help your customer to solve the problems experienced, I would appreciate if you could send me further details about the protocol used by the customer.

Which primary antibody was used? Could you please provide further details as the host species, the isotype, etc.. And which species were used?

If this isotype control is used as a negative control, as it is not specific to any target it shouldn’t detect any protein, therefore, it shouldn’t give any signal. Why are you expecting signal anyway?

Thank you for your cooperation. I look forward to receiving your reply.

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Question
Answer

Thank you for contacting us. My colleague is out today but we did recieve information regarding your enquiry so I thought I would pass than on to you. According to the lab, we do not quantify the purity of this product. The product is purified from ascites fluid by Protein G chromotatography, and the concentration is determined using spectrophotometer using the product after purification. I hope this information is of help to you. Please let me know if there is anything else I can assist you with. Thank you Regards, Michelle Hong Technical Support Specialist Thermo Fisher Scientific 46117 Landing Parkway Fremont, CA 94539

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Question
Answer

Thank you for contacting Abcam. The only information that I can find out about the purity of ab81032 is that it was purified from ascites fluid by Protein G chromatogrphy, I cannot find out any information about how pure the final product is. I am sorry that I cannot provide more information, but if there is anything else I can help you with, please let me know.

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