Mouse IgG1 kappa [MOPC-21] (Allophycocyanin) - Isotype Control (ab239462)

Overview

  • Product name

    Mouse IgG1 kappa [MOPC-21] (Allophycocyanin) - Isotype Control
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity

    ab239462 has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • Clonality

    Monoclonal
  • Clone number

    MOPC-21
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab239462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab239462 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

Images

  • Example of non-specific ab239462 signal on human peripheral blood; surface staining, 3 μg/ml.

References

ab239462 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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