Product nameAnti-Mouse IgG2a antibody [RM107]
See all Mouse IgG2a primary antibodies
DescriptionRabbit monoclonal [RM107] to Mouse IgG2a
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IP, ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to Mouse IgG2a.
- Mouse IgG2a kappa
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesProtein A affinity purified from an animal origin-free and protein-free culture supernatant.
Our Abpromise guarantee covers the use of ab190463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml.
Non-reducing conditions only
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
- Ig gamma-2A chain C region, A allele antibody
- Igg2a antibody
- Ighg antibody
All lanes : Anti-Mouse IgG2a antibody [RM107] (ab190463) at 0.2 µg/ml
Lane 1 : Mouse IgG2a kappa (non-reducing conditions)
Lane 2 : Mouse IgG2a kappa (reducing conditions)
Lysates/proteins at 0.02 µg per lane.
ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ab190463 has not yet been referenced specifically in any publications.