For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Full length protein corresponding to Mouse IgG2a.
Our Abpromise guarantee covers the use of ab190463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 0.5 - 2 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml.
Non-reducing conditions only
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
ELISA analysis of mouse immunoglobulins labeling the Fab region of mouse IgG2a using ab190463 at 200 ng/ml, 50 ng/ml, or 10 ng/ml. The plate was coated with 50 ng/well of different immunoglobulins. No cross reactivity with IgG2aλ, IgG1, IgG3, IgM, IgA, IgE, Human IgG, rat IgG, or goat IgG was detected. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA analysis of mouse IgG2a using ab190463 at different concentrations. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ab190463 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"