Recombinant

Recombinant Anti-Mouse IgG2a antibody [RM219] (Biotin) (ab222764)

Overview

  • Product name

    Anti-Mouse IgG2a antibody [RM219] (Biotin)
    See all Mouse IgG2a primary antibodies
  • Description

    Rabbit monoclonal [RM219] to Mouse IgG2a (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    ab222764 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Full length protein corresponding to Mouse IgG2a.

  • Positive control

    • WB: Non-reduced mouse IgG2a

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab222764 was purified from an animal origin–free culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    RM219
  • Isotype

    IgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab222764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml.
ELISA Use a concentration of 0.005 - 0.2 µg/ml.

Target

Images

  • All lanes : Anti-Mouse IgG2a antibody [RM219] (Biotin) (ab222764) at 0.5 µg/ml

    Lane 1 : Non-reduced mouse IgG2a
    Lane 2 : Reduced mouse IgG2a


    This antibody reacts to non-reduced IgG2a ~150 kDa.

  • A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

  • ELISA of mouse immunoglobulins shows ab222764 reacts to the Fc region of mouse IgG2a; no cross  reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

References

ab222764 has not yet been referenced specifically in any publications.

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