Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM219] to Mouse IgG2a (Biotin)
- Suitable for: WB, ELISA
- Reacts with: Mouse
- Conjugation: Biotin
Product nameAnti-Mouse IgG2a antibody [RM219] (Biotin)
See all Mouse IgG2a primary antibodies
DescriptionRabbit monoclonal [RM219] to Mouse IgG2a (Biotin)
Specificityab222764 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to Mouse IgG2a.
- WB: Non-reduced mouse IgG2a
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Purification notesab222764 was purified from an animal origin–free culture supernatant.
Our Abpromise guarantee covers the use of ab222764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml.|
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
- Ig gamma-2A chain C region, A allele antibody
- Igg2a antibody
- Ighg antibody
All lanes : Anti-Mouse IgG2a antibody [RM219] (Biotin) (ab222764) at 0.5 µg/ml
Lane 1 : Non-reduced mouse IgG2a
Lane 2 : Reduced mouse IgG2a
This antibody reacts to non-reduced IgG2a ~150 kDa.
A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
ELISA of mouse immunoglobulins shows ab222764 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab222764 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
ab222764 has not yet been referenced specifically in any publications.