Overview

  • Product name

    Mouse IgG2a ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low IgG2a 16 43.4ng/ml 1.8%
    Medium IgG2a 16 69.8ng/ml 1.2%
    High IgG2a 16 112ng/ml 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Low IgG2a 8 41.4ng/ml 4.6%
    Medium IgG2a 8 67.4ng/ml 4.8%
    High IgG2a 8 110ng/ml 4.1%
  • Sample type

    Serum, Tissue Culture Media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    318.8 pg/ml
  • Range

    7.81 ng/ml - 250 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 98.4 % - %
    Tissue Culture Media = 98.2 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s Mouse IgG2a in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse IgG2a in Tissue Culture Media and Serum.

    IgG2a specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG2a detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG2a amount of sample captured in plate.

  • Notes

    IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG2a apart from its IgG counterparts. Properties of IgG2a include neutralization of toxins and diffusion in the extracellular space.

    Cross Reactivity

    IgG% Cross Reactivity
    mouse IgG2a100
    mouse IgG30.49
    mouse IgM0.12
    mouse IgG1<0.01
    mouse IgG2b<0.01
    rat IgG2a<0.01
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133046.

Protocols

References

This product has been referenced in:

  • Hu B  et al. Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus. Virol J 11:142 (2014). Read more (PubMed: 25107552) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.
We have not specifically tested rabbit but I doubt it would cross react. We tested rat IgG2a in this kit and it only had 0.01% cross reactivity. Generally speaking, you typically should not get significant cross reactivity between mouse immunoglobulins and other species IgG’s. Thank you for your email and have a great day!

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