Mouse IgG2a, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191)

Overview

  • Product name
    Mouse IgG2a, kappa monoclonal [18C8BC7AD10] - Isotype Control
  • Specificity
    This Mouse IgG2a Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
  • Tested applications
    Suitable for: Flow Cyt, ICCmore details
  • General notes

    This antibody clone is manufactured by Abcam.

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.

    This product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Monoclonal
  • Clone number
    18C8BC7AD10
  • Isotype
    IgG2a
  • Light chain type
    kappa

Applications

Our Abpromise guarantee covers the use of ab170191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Images

  • Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG2a Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).

    An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

  • Flow Cytometry

    Flow cytometry experiments with 4% PFA fixed Hela (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG2a Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

  • Fluorescence ELISA.

    An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG2a Isotype Control Antibody and detecting with Alexa Fluor® conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

References

This product has been referenced in:
  • Chen HQ & Gao D Inhibitory effect of microRNA-154 targeting WHSC1 on cell proliferation of human skin squamous cell carcinoma through mediating the P53 signaling pathway. Int J Biochem Cell Biol 100:22-29 (2018). WB ; Human . Read more (PubMed: 29727714) »
  • Foss CA  et al. SPECT/CT Imaging of Mycobacterium tuberculosis Infection with [125I]anti-C3d mAb. Mol Imaging Biol N/A:N/A (2018). Read more (PubMed: 29998399) »
See all 4 Publications for this product

Customer reviews and Q&As

There are currently no Questions for ab170191.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up