Mouse IgG2a, kappa monoclonal [18C8BC7AD10] - Isotype Control - BSA and Azide free (ab221849)

Overview

  • Product name

    Mouse IgG2a, kappa monoclonal [18C8BC7AD10] - Isotype Control - BSA and Azide free
    See all Coiled-coil domain-containing protein 113 isotype controls
  • Specificity

    This Mouse IgG2a Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
  • Tested applications

    Suitable for: Flow Cyt, ICCmore details
  • General notes

    This antibody clone is manufactured by Abcam.

    Ab221849 is a PBS only buffer formulated version of ab170191.

    Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.

     

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Monoclonal
  • Clone number

    18C8BC7AD10
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Alternative names

    • CCDC113
    • Coiled-coil domain-containing protein 113 Gene names
    • DKFZp434N1418
    • HSPC065

Applications

Our Abpromise guarantee covers the use of ab221849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

Images

  • This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170191).

    Flow cytometry experiments with 4% PFA fixed Hela (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG2a Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

References

This product has been referenced in:

  • Arslan E  et al. Glycosaminoglycan-mimetic signals direct the osteo/chondrogenic differentiation of mesenchymal stem cells in a three-dimensional peptide nanofiber extracellular matrix mimetic environment. Biomacromolecules N/A:N/A (2016). Flow Cyt ; Rat . Read more (PubMed: 26840042) »
  • Olsen JB  et al. G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports 7:454-70 (2016). ChIP ; Zebrafish . Read more (PubMed: 27546533) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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