Product nameMouse IgG2a, kappa monoclonal [18C8BC7AD10] - Isotype Control - BSA and Azide free
See all Coiled-coil domain-containing protein 113 isotype controls
SpecificityThis Mouse IgG2a Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
Tested applicationsSuitable for: Flow Cyt, ICCmore details
This antibody clone is manufactured by Abcam.
Ab221849 is a PBS only buffer formulated version of ab170191.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Light chain typekappa
- Coiled-coil domain-containing protein 113 Gene names
Our Abpromise guarantee covers the use of ab221849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 1 µg/ml.|
This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170191).
Flow cytometry experiments with 4% PFA fixed Hela (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG2a Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
This product has been referenced in:
- Arslan E et al. Glycosaminoglycan-mimetic signals direct the osteo/chondrogenic differentiation of mesenchymal stem cells in a three-dimensional peptide nanofiber extracellular matrix mimetic environment. Biomacromolecules N/A:N/A (2016). Flow Cyt ; Rat . Read more (PubMed: 26840042) »
- Olsen JB et al. G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports 7:454-70 (2016). ChIP ; Zebrafish . Read more (PubMed: 27546533) »