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  1. Link

    mouse-igg2a-kappa-monoclonal-b128-isotype-control-ab91361.pdf

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Isotype/Loading Controls Isotype Controls Mouse
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Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

  • Datasheet
  • SDS
Submit a question References (7)

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Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

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Overview

  • Product name

    Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control
    See all Mouse isotype controls
  • Tested applications

    Suitable for: Flow Cytmore details
  • Immunogen

    Synthetic peptide. Hapten from sequence of plant origin.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from supernatant from an in vitro culture.
  • Clonality

    Monoclonal
  • Clone number

    B12/8
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Alternative Versions

    • CF405M Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab126036)
    • APC Mouse IgG2a [B12/8] - Isotype Control (ab91364)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells. ab91361 is a negative isotype control
Notes
Flow Cyt
Use 1µg for 106 cells. ab91361 is a negative isotype control

Images

  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
    Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

    Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg. 

  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
    Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

    Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab91361? Please let us know so that we can cite the reference in this datasheet.

ab91361 has been referenced in 7 publications.

  • Benz PM  et al. AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting. Acta Physiol (Oxf) 228:e13325 (2020). PubMed: 31162891
  • Chen X  et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
  • Rios DA  et al. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis. Sci Rep 7:13283 (2017). PubMed: 29038590
  • Khan A  et al. Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy. Sci Rep 7:15010 (2017). PubMed: 29118429
  • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . PubMed: 25969669
  • Jun E  et al. Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA. PLoS One 10:e0118310 (2015). PubMed: 25705892
  • Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014). Flow Cyt . PubMed: 24573683

Customer reviews and Q&As

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