Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

Submit a question References (7)
Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

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APC CF405M

Overview

  • Product name

    Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control
    See all Mouse isotype controls

  • Tested applications

    Suitable for: Flow Cytmore details

  • Immunogen

    Synthetic peptide. Hapten from sequence of plant origin.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from supernatant from an in vitro culture.

  • Clonality

    Monoclonal

  • Clone number

    B12/8

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells. ab91361 is a negative isotype control
Notes
Flow Cyt
Use 1µg for 106 cells. ab91361 is a negative isotype control

Images

  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
    Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

    Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg. 

  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
    Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

    Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

References (7)

Publishing research using ab91361? Please let us know so that we can cite the reference in this datasheet.

ab91361 has been referenced in 7 publications.

  • Benz PM  et al. AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting. Acta Physiol (Oxf) 228:e13325 (2020). PubMed: 31162891
  • Chen X  et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
  • Rios DA  et al. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis. Sci Rep 7:13283 (2017). PubMed: 29038590
  • Khan A  et al. Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy. Sci Rep 7:15010 (2017). PubMed: 29118429
  • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . PubMed: 25969669
  • Jun E  et al. Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA. PLoS One 10:e0118310 (2015). PubMed: 25705892
  • Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014). Flow Cyt . PubMed: 24573683

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Q&A
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