Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
Overview
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Product name
Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control
See all Mouse isotype controls -
Specificity
ab18415 MG2a-53 immunoglobulin reacts with KLH. The MG2a-53 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues -
Tested applications
Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WBmore details -
Immunogen
Trinitrophenol + KLH
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General notes
ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Isotype control notes
ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. -
Clonality
Monoclonal -
Clone number
MG2a-53 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| IHC-Fr |
Use at an assay dependent dilution.
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| Flow Cyt |
Use at an assay dependent dilution.
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| IP |
Use at an assay dependent dilution.
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| WB |
Use at an assay dependent dilution.
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| Notes |
|---|
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IHC-P
Use at an assay dependent dilution. |
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IHC-Fr
Use at an assay dependent dilution. |
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Flow Cyt
Use at an assay dependent dilution. |
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IP
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab18415 has been referenced in 9 publications.
- Eylert G et al. Skin regeneration is accelerated by a lower dose of multipotent mesenchymal stromal/stem cells-a paradigm change. Stem Cell Res Ther 12:82 (2021). PubMed: 33494813
- Folly AJ et al. Detection of Usutu virus infection in wild birds in the United Kingdom, 2020. Euro Surveill 25:N/A (2020). PubMed: 33063656
- Modasia A et al. Regulation of Enteroendocrine Cell Networks by the Major Human Gut Symbiont Bacteroides thetaiotaomicron. Front Microbiol 11:575595 (2020). PubMed: 33240233
- Perera ON et al. Telomerase promotes formation of a telomere protective complex in cancer cells. Sci Adv 5:eaav4409 (2019). PubMed: 31616780
- Li H et al. Development of a macrophage-targeting and phagocytosis-inducing bio-nanocapsule-based nanocarrier for drug delivery. Acta Biomater 73:412-423 (2018). PubMed: 29673839
- Dessilly G et al. ABCB1 1199G>A genetic polymorphism (Rs2229109) influences the intracellular accumulation of tacrolimus in HEK293 and K562 recombinant cell lines. PLoS One 9:e91555 (2014). ICC/IF . PubMed: 24621983
- Perry MC et al. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Mol Cell Biol 34:4232-43 (2014). PubMed: 25246633
- Veith C et al. Paxillin regulates pulmonary arterial smooth muscle cell function in pulmonary hypertension. Am J Pathol 181:1621-33 (2012). IHC-P . PubMed: 22959909
- Koskinen LL et al. Association study of the IL18RAP locus in three European populations with coeliac disease. Hum Mol Genet 18:1148-55 (2009). PubMed: 19103669