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Isotype/Loading Controls Isotype Controls Mouse
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Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

  • Datasheet
  • SDS
Q&A (1)References (5)

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Overview

  • Product name

    Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control
    See all Mouse isotype controls
  • Specificity

    ab18415 MG2a-53 immunoglobulin reacts with KLH. The MG2a-53 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WBmore details
  • Immunogen

    Trinitrophenol + KLH

  • General notes


    ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store undiluted.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Isotype control notes

    ab18415 MG2a-53 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.
  • Clonality

    Monoclonal
  • Clone number

    MG2a-53
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Flow Cyt
Use at an assay dependent dilution.
IP
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
Notes
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Flow Cyt
Use at an assay dependent dilution.
IP
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab18415? Please let us know so that we can cite the reference in this datasheet.

    ab18415 has been referenced in 5 publications.

    • Li H  et al. Development of a macrophage-targeting and phagocytosis-inducing bio-nanocapsule-based nanocarrier for drug delivery. Acta Biomater 73:412-423 (2018). PubMed: 29673839
    • Dessilly G  et al. ABCB1 1199G>A genetic polymorphism (Rs2229109) influences the intracellular accumulation of tacrolimus in HEK293 and K562 recombinant cell lines. PLoS One 9:e91555 (2014). ICC/IF . PubMed: 24621983
    • Perry MC  et al. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Mol Cell Biol 34:4232-43 (2014). PubMed: 25246633
    • Veith C  et al. Paxillin regulates pulmonary arterial smooth muscle cell function in pulmonary hypertension. Am J Pathol 181:1621-33 (2012). IHC-P . PubMed: 22959909
    • Koskinen LL  et al. Association study of the IL18RAP locus in three European populations with coeliac disease. Hum Mol Genet 18:1148-55 (2009). PubMed: 19103669

    Customer reviews and Q&As

    Q&A
    Submit a question

    Question

    Could you please let me know if you have mouse IgG2a isotype control sodium azide free available?

    Thank you heaps J

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 06 2012

    Answer

    Thank you for contacting us.


    Please check the following link

    https://www.abcam.com/Mouse-IgG2a-kappa-MOPC-173-low-endotoxin-isotype-control-ab18449.html

    https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18414.html

    https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18415.html

    https://www.abcam.com/Mouse-IgG2a-ICIGG2A-Isotype-Control-ab91361.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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