Mouse IgG2a, kappa monoclonal [MG2a-53] (Low endotoxin, Azide Free) - Isotype control  (ab18414)

Overview

  • Product name
    Mouse IgG2a, kappa monoclonal [MG2a-53] (Low endotoxin, Azide Free) - Isotype control 
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Functional Studies, Flow Cyt, WB, IP, IHC-Fr, IHC-Pmore details
  • Immunogen

    Chemical/ Small Molecule (Trinitrophenol) conjugated to KLH.

  • General notes


    ab18414, MG2a-53 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Ab18414 is 'LEAF Purified'. This is a low endotoxin azide free formulation containing no preservative. The antibody is 0.2 µm filter sterilized and the endotoxin level is <0.01 ng/µg protein.
  • Isotype control notes
    ab18414, MG2a-53 immunoglobulin reacts with KLH and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality
    Monoclonal
  • Clone number
    MG2a-53
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

References

This product has been referenced in:
  • Munsie LN  et al. Retromer-dependent neurotransmitter receptor trafficking to synapses is altered by the Parkinson's disease VPS35 mutation p.D620N. Hum Mol Genet 24:1691-703 (2015). Read more (PubMed: 25416282) »
  • Su SC  et al. Annexin 2 Regulates Endothelial Morphogenesis by Controlling AKT Activation and Junctional Integrity. J Biol Chem 285:40624-34 (2010). Read more (PubMed: 20947498) »
See all 2 Publications for this product

Customer reviews and Q&As

1-9 of 9 Q&A

Answer

I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Abcam also carries other products essential for Flow Cytometry such as isotype controls which confirm that the primary antibody binding is specific and not results of non-specific Fc receptor binding or other protein interactions. The isotype control should match the species, isotype, clonality and conjugation of the primary antibody. In this case the isotype control ab56670 is: Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414) and for ab60719: Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control (ab18447). More information about these isotype controls may be found at the following locations: Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18414): https://www.abcam.com/mouse-igg2a-kappa-monoclonal-mg2a-53-isotype-control-ab18414.html Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control (ab18447): https://www.abcam.com/mouse-igg1-kappa-monoclonal-mg1-45-isotype-control-ab18447.html If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit. Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html 7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4oC for a few weeks should not significantly affect the staining. However, we would recommend to follow the individual storage instructions provided on the individual datasheets as soon as possible after delivery. This will help to ensure that the antibody remains stable and that we can continue to provide our guarantee.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab54426 used in IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

With regards to the isotype controls:
For ab2213, the light chain type is unknown, so I would recommend to use a general IgG2a isotype control rather than a specific IgG kappa chain isotype control. For example the following product:

ab91361 Mouse IgG2a [ICIGG2A] - Isotype Control
https://www.abcam.com/index.html?datasheet=91361 https://www.abcam.com/index.html?datasheet=91361.

However, please note this is not tested in IHC-P. Unfortunately, I am sorry we do not have any suitable isotype controls tested in IHC-P in this case.

ab27478 is a suitable isotype control for ab18981
ab91353 is a suitable isotype control for ab54426

Any feedback that you can provide regarding ab54426 will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

We do not currently have a pan- mouse IgG isotype control suitable for in vivo use on mouse. We do have isotype controls for each IgG subtype which have beenLEAF Purified. This is a low endotoxin azide free formulation containing no preservative and is 0.2 µm filter sterilized the endotoxin level for these products is <0.01 ng/µg protein. Further these have beenscreened on a variety of resting, activated, live, and fixed mouse tissues.

ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype controlhttps://www.abcam.com/Mouse-IgG1-kappa-monoclonal-MG1-45-Isotype-control-ab18447.html

ab18414Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype controlhttps://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18414.html

ab18457Mouse IgG2b, kappa monoclonal [MPC-11] - isotype control. BSA/Azide freehttps://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MPC-11-isotype-control-BSA-Azide-free-ab18457.html

ab18392Mouse IgG3, kappa monoclonal [MG3-35] - Azide freehttps://www.abcam.com/Mouse-IgG3-kappa-monoclonal-MG3-35-Azide-free-ab18392.html

Should you be interested in these products at the volumes which you have specified these would be considered bulk purchases and may qualify for special pricing. Please contact sales@abcam.com for more information about this.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.


Please check the following link

https://www.abcam.com/Mouse-IgG2a-kappa-MOPC-173-low-endotoxin-isotype-control-ab18449.html

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18414.html

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18415.html

https://www.abcam.com/Mouse-IgG2a-ICIGG2A-Isotype-Control-ab91361.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Clone MG2a-53 (mouse IgG2a isotype control) is originally derived from BALB/c.

Read More

Answer

Thank you for your enquiry. We do not have specific data on the half-life of this antibody, but I can give you some numbers of rat IgG2b half-life based on literature. These references refer to natural IgG2 with a half-life of 20-30 days in blood. http://www3.interscience.wiley.com/cgi-bin/abstract/106594991/ABSTRACT http://www.ncbi.nlm.nih.gov/books/bv.fcgi?highlight=half-life,antibody&rid=imm.figgrp.471 This reference measured radioactive-labeled antibodies in rat blood, found much shorter half-lives. IgG2b was not measured. http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&uid=7429545&cmd=showdetailview&indexed=google Of course, various factors will contribute to IgG2b clearance, such as: number of available targets antigens, Fc receptor binding, stability of antibody, age of the rats, etc.

Read More

Answer

Thank you for getting back to me. If you provide me with the details of your most recent order of your primary and secondary antibodies (order number and date) I will do my best to cancel the order before it is shipped and provide you with credit to the value of the order. If you have received the antibodies on receipt of this e-mail you can send them back in the same packaging that you received the antibodies in and you will be reimbursed (minus a 20% restocking fee). Please return them with the same ice packs that were in the box during shipping (refrozen). I am in contact with the source of this antibody and I will be in touch when I have further information as to the availability of a new batch. I appreciate your patience in this matter.

Read More

Question

Hi, Several weeks ago, I've bought one of your mouse monoclonal antibody (aE11 to C5b-9). As a negative control, I've also bought an isotype control (mouse IgG2a kappa monoclonal (MG2a-53)) and a Goat polyclonal antibody to Mouse IgG PE (ab7002) as a secondary antibody. The purpose of my study is to detect C5b-9 complexes on different cells (lymphocytes, monocytes, etc.) in patients with an inflammatory disease and in controls (by flowcytometry). As positive controls, I activate the complement system by different ways (so we can normally see more C5b-9 on the cells' surface when the complement is activated). At the beginning of my experiment, I've seen many significant differences between the different conditions used (patients, controls and positive controls) whereas now, I can't see those results anymore. The results I obtained with the isotype control are even higher than those with the regular monoclonal Ab; more C5b-9 complexes are present on the cells WITHOUT using the monoclonal antibody. I've noted that this event correlates with the fact that I now use new primary (lot # 166383) and secondary (154790) antibodies. I've received those new Abs less than 3 weeks ago. I first guessed that I might have diluted the secondary Ab more, so I've tried many dilutions (1:5, 1:100, 1:200): No positive results. What dilution of each Ab do you recommended to use in flowcytometry? What do you think the problem could be? Could it be because of the new Abs I use? (Which one?). What do you suggest? Is it possible to send me new Abs (different lot numbers maybe...)? Thank you for your help, Regards,

Read More
Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody, your enquiry definitely sounds batch related. The fact that you have been obtaining negative results in your FACS system until the arrival of the most recent batch suggests that the problem is with the new primary antibody that you have received. Unfortunately at the moment we only have batch # 166383 in stock. However, I would like to offer you a credit note that you can use against the cost of another Abcam antibody. This is provided that the antibody was purchased within the past 90 days. If this is the case please e-mail me the date of purchase and the original purchase order number. I look forward to hearing from you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up