Product nameMouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
See all Mouse isotype controls
DescriptionMouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
SpecificityThis Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WB, ChIP/Chip, ChIPmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Light chain typekappa
- Mouse Isotype Control
Our Abpromise guarantee covers the use of ab18413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|ChIP/Chip||Use at an assay dependent concentration. PubMed: 18636108|
|ChIP||Use at an assay dependent concentration. PubMed: 18636108|
ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.
This product has been referenced in:
- Bedenbender K et al. Inflammation-mediated deacetylation of the ribonuclease 1 promoter via histone deacetylase 2 in endothelial cells. FASEB J 33:9017-9029 (2019). Read more (PubMed: 31039328) »
- Hu T et al. Neuroprotection induced by Navß2-knockdown in APP/PS1 transgenic neurons is associated with NEP regulation. Mol Med Rep 20:2002-2011 (2019). Read more (PubMed: 31257483) »