Mouse IgG2a, Kappa Monoclonal [MOPC-173] Isotype Control- ChIP Grade (ab18413)

Overview

  • Product name
    Mouse IgG2a, Kappa Monoclonal [MOPC-173] Isotype Control- ChIP Grade
    See all Mouse isotype controls
  • Specificity
    This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, WB, ChIP/Chip, ChIPmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab18413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP/Chip Use at an assay dependent concentration. PubMed: 18636108
ChIP Use at an assay dependent concentration. PubMed: 18636108

Images

  • ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.

References

This product has been referenced in:
  • Lian SL  et al. A SNP uncoupling Mina expression from the TGFß signaling pathway. Immun Inflamm Dis 6:58-71 (2018). Read more (PubMed: 28967702) »
  • Song L  et al. Hippocampal PPARa is a novel therapeutic target for depression and mediates the antidepressant actions of fluoxetine in mice. Br J Pharmacol 175:2968-2987 (2018). Read more (PubMed: 29722018) »
See all 20 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Question
Answer

Thank you for your reply.

They are the same antibody (same clone), just that ab18449 has been screened for low endotoxin levels for use with cell culture.

I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting us.

The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

Therefore, you should use the mouse control IgG2a ab18413 and NOT the rabbit IgG ab37415.

To learn more about isotype controls, please see the link below:

https://www.abcam.com/index.html?pageconfig=technicalfaqs#5

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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