Mouse IgG2a, kappa monoclonal [MOPC-173] - Isotype Control (Low endotoxin, Azide free) (ab18449)

Overview

  • Product name
    Mouse IgG2a, kappa monoclonal [MOPC-173] - Isotype Control (Low endotoxin, Azide free)
    See all Mouse isotype controls
  • Specificity
    This clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, Flow Cyt, ICC/IF, IP, WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes
    The ab18449 (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    MOPC-173
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Question
Answer

Thank you for your reply.

The antibody format is phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 0.1 EU/μg of the protein (< 0.01 ng/μg of the protein) as determined by the LAL test.

I hope this information helps. Please contact us with any other questions.

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Question
Answer

Thank you for your reply.

They are the same antibody (same clone), just that ab18449 has been screened for low endotoxin levels for use with cell culture.

I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting us.

The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

Therefore, you should use the mouse control IgG2a ab18413 and NOT the rabbit IgG ab37415.

To learn more about isotype controls, please see the link below:

https://www.abcam.com/index.html?pageconfig=technicalfaqs#5

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.


Please check the following link

https://www.abcam.com/Mouse-IgG2a-kappa-MOPC-173-low-endotoxin-isotype-control-ab18449.html

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18414.html

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MG2a-53-Isotype-control-ab18415.html

https://www.abcam.com/Mouse-IgG2a-ICIGG2A-Isotype-Control-ab91361.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank youfor your inquiry.

I can confirm that we do have three anti NS1 antibodies for Dengue virus in our catalog..

Isotype control are not included and have to be purchased separately.

ab41616 (Anti-Dengue Virus NS1 glycoprotein antibody [DN3]):

https://www.abcam.com/index.html?datasheet=41616 (or use the following: https://www.abcam.com/index.html?datasheet=41616).

Heaton NS et al. Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis. Proc Natl Acad Sci U S A 107:17345-50 (2010). ICC/IF. PubMed: 20855599

Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

ab41623 (Anti-Dengue Virus NS1 glycoprotein antibody [DN2]):

https://www.abcam.com/index.html?datasheet=41623 (or use the following: https://www.abcam.com/index.html?datasheet=41623).

Smith KM et al. Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype. Virol J 8:289 (2011). IF. PubMed: 21658241

Isotype control: ab18447 (Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control)
https://www.abcam.com/index.html?datasheet=18447 (or use the following: https://www.abcam.com/index.html?datasheet=18447).

ab41490 (Anti-Dengue Virus NS1 glycoprotein antibody [DN1]):

https://www.abcam.com/index.html?datasheet=41490 (or use the following: https://www.abcam.com/index.html?datasheet=41490).

Currently we do not know of any references using this antibody.

Isotype control: ab18449 (Mouse IgG2a, kappa [MOPC-173] (low endotoxin) - isotype control)
https://www.abcam.com/index.html?datasheet=18449 (or use the following: https://www.abcam.com/index.html?datasheet=18449).

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at leastsix monthfrom the date of purchase when stored correctly.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

We do not sell directly to Pakistan. Please contact our trusted distributor for pricing information:

Friends Scientific Corporation
3-B Ali Continental,
1-Mozang Road
Lahore
54000
Pakistan

Tel 4237236176 Fax 4237321500 Email mailto:friends1@wol.net.pk

I hope this information is helpful and wish you good luck with your experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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