Mouse IgG2a, monoclonal (FITC) - Isotype control (ab81197)

Overview

  • Product name

    Mouse IgG2a, monoclonal (FITC) - Isotype control
    See all Mouse isotype controls
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Conjugation notes

    Excitation: 488 nm. Peak emission: 525 nm.
  • Tested applications

    Suitable for: Flow Cytmore details
  • General notes

    Reagents should be brought to room temperature (22 ± 3°C) before use. Protect cells from light sources during incubation with antibodies and prior to analysis.

Properties

Applications

Our Abpromise guarantee covers the use of ab81197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.

References

This product has been referenced in:

  • Lydon H  et al. Peripheral mononuclear blood cell apheresis in a preclinical ovine model. BMC Vet Res 14:47 (2018). Read more (PubMed: 29439735) »
  • Santoro A  et al. Relationship between CK19 expression, deregulation of normal keratinocyte differentiation pattern and high risk-human papilloma virus infection in oral and oropharyngeal squamous cell carcinoma. Infect Agent Cancer 10:46 (2015). Flow Cyt . Read more (PubMed: 26672675) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Answer

Thank you for contacting us.
I can confirm the isotype controls suggested are suitable for the antibodies requested.
The difference in price between ab91353 and ab81197 lies in the volume difference (the volume of ab91353 doubles ab81197’s volume) and the manufacturing and purification process often make price vary between what seems to be similar products.
I hope this helps. Please do not hesitate to contact us if you require further assistance.

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Answer

Thank you for your reply.

Although the exact amount of FITC in ab58864 and ab81197 has not been determined, for the purposes of your calculations, you can assume that it is 40x greater.

Both ab16892 and ab58864, are the same clone of antibody, but the ab58864 has a FITC label. You could also use ab12121:

https://www.abcam.com/TLR9-antibody-26C593-ab12121.html

If there is anything else I can help you with, please let me know.

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Answer

Thank you for contacting us.

Ab81197 Mouse IgG2a (FITC) - Isotype control is supplied in a500 µl size at 4mg/ml. To correctly use this control you will need to use this at the same concentration as yourab58864 Anti-TLR9 antibody [5G5] (FITC) which is supplied at 0.1mg/ml. You will therefore need 40x less of the isotype control than you use for the TLR9 antibody.

We do carry a number of alternative TLR9 antibodies many of which are guaranteed to work in Flow Cytometry. The entire list may be found at the following link:

https://www.abcam.com/index.html?t=73449&pt=1

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Hello! Yes, this product is in stock. You can also search our website for the catalog number and the top of the datasheet will tell you whether it is in stock. Please let me know if you have any further questions!

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Question

                    Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti-CD200 antibody      unconjugated    ab17225 Anti-CD146 antibody      PE                           ab78488 Anti-CD34 antibody         unconjugated    ab6330 Anti-CD71 antibody         unconjugated    ab117720 Anti-CD24 antibody         FITC                       ab30350 Anti-CD73 antibody         unconjugated    ab81720                                                 PE                           ab106697 Anti-CD105 antibody      PE                           ab91238                                                 Unconjugated   ab11414 Anti-Cd45 antibody         PE                           ab90960 Anti-STRO1 antibody      unconjugated    ab102969 Anti-CD90 antibody         unconjugated    ab23894                                                 FITC                       ab11155 Anti-CD14 antibody         FITC                       ab86896               Anti-cytokeratin 15         unconjugated    ab1385 IgG2a Isotype control     FITC                       ab81197   Looking forward to your soonest reply. Thank you.   Best regards, Penny Hor BITA LIFESCIENCE SDN. BHD. C-23-05, Dataran 32, No. 2, Jalan 19/1, 46300 Petaling Jaya, Selangor Darul Ehsan, Malaysia t +603 7960 8286    e info@bitalifesc.com.my f +603 7960 8262     

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Answer

Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not been determined. In these instances, it is probably that the light chain sub-class is kappa, given that murine serum immunoglobulins are 95% kappa and 5% lambda, as discussed in the reference at the following link: http://www.ncbi.nlm.nih.gov/pubmed/3116408 Mol Immunol. 1987 Jul;24(7):751-7. PMID:3116408 Of the antibodies your customer has enquired about, The light chain sub-class has been determined for three: ab90960, ab86896, and ab1385. All are kappa light chain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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