Overview

  • Product name

    Mouse IgG2b ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse IgG2b 16 59.4ng/ml 6.2%
    Mouse IgG2b 16 112ng/ml 5.2%
    Mouse IgG2b 16 208ng/ml 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Mouse IgG2b 8 53.7ng/ml 6.7%
    Mouse IgG2b 8 104ng/ml 5.4%
    Mouse IgG2b 8 201ng/ml 7.9%
  • Sample type

    Serum, Tissue Culture Media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 1.05 ng/ml
  • Range

    7.81 ng/ml - 250 ng/ml
  • Recovery

    = 101.5 %

    Sample specific recovery
    Sample type Average % Range
    Serum 98.3 % - %
    Tissue Culture Media 104.6 % - %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s IgG2b mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IgG2b in mouse tissue culture media and serum.

    IgG2b specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated IgG2b detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a colored product that changes after adding acidic stop solution. The density of coloration is directly proportional to the IgG2b amount of sample captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Assay Buffer 13 Concentrate 1 x 45ml
    20X Wash Buffer Concentrate 1 x 100ml
    Anti-mouse IgG2b - HRP Conjugate 1 x 5ml
    Goat anti-mouse IgG Microplate (12 x 8 wells) 1 unit
    Mouse IgG2b lyophilized Standard 2 vials
    Plate Sealer 2 units
    Stop Solution 2 1 x 10ml
    TMB Substrate 1 x 10ml
  • Research areas

  • Relevance

    IgG2 is divided into two subclasses; IgG2a and IgG2b. It is a glycoprotein which consists of two identical heavy chains (50 kDa each) and two identical light chains (25 kDa each), to give a combined mass of approximately 150 kDa. The chains are held in place by covalent disulfide bonds. Each light chain contains two immunoglobulin (Ig) domains, while the heavy chains contain four Ig domains each. In the middle of each heavy chain is a relative varying portion called the “hinge region” which is unique to each IgG. This region allows for molecular flexibility and sets IgG apart from its IgG counter-parts. Properties of IgG2b include neutralization of toxins and diffusion in the extracellular space.
  • Cellular localization

    Isoform 1: Cell membrane; Single-pass membrane protein. Isoform 2: Secreted.
  • Alternative names

    • Gamma2b
    • Ig gamma-2B chain C region
    • IgG2b
    • IGG2B_MOUSE
    • Igh 3
    • Immunoglobulin heavy constant gamma 2B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab136941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab136941.

Protocols

References

ab136941 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab136941.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up