Product nameMouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free
See all Coiled-coil domain-containing protein 113 isotype controls
SpecificityThis Mouse IgG2b Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
This antibody clone is manufactured by Abcam.
ab221850 is a PBS only buffer formulated version of ab170192.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Light chain typekappa
- Coiled-coil domain-containing protein 113 Gene names
Our Abpromise guarantee covers the use of ab221850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 1 µg/ml.|
This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170192).
Flow cytometry experiments with 4% PFA fixed Hela (human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast)were performed with the IgG2b Isotype Control Antibody (red) and no primary antibody negative control (black). An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody.
The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
ab221850 has not yet been referenced specifically in any publications.