Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control (ab18469)

Overview

  • Product name
    Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt, IPmore details
  • Immunogen

    Trinitrophenol and KLH.

  • General notes
    Isotype control has been screened on a variety of resting, activated, live, and fixed rat and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Monoclonal
  • Clone number
    MPC-11
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
WB
Flow Cyt
IP
  • Application notes
    Flow Cyt: Use at an assay dependent dilution for surface and intracellular staining.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References

    ab18469 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Q&A

    Answer

    Thank you for contacting Abcam.

    We have not tested in-house reactivity of ab18469 against KLH, so I cannot say for certain whether the antibody will or will not bind to the KLH tag.

    We have also not epitope mapped these antibodies, so we cannot say exactly where they bind, but I would suspect that they bind to different regions.

    Please let me know if there is anything else I can help you with.

    Read More

    Answer

    Thank you for your enquiry.


    Both you capture and detection antibodies should recognize these isotype controls. However, as these products have not been tested in sandwich ELISA we do not know whether the capture antibody will recognize the same site or otherwise interfere with the detection antibody. Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. As these products have not been tested in this application and they have not been tested against one anotherI cannot recommend one isotype control over the other.

    Regarding the clone numbers, these are created by the originator the antibody for identification purposes. The epitopes of these products have not been mapped to our knowledge.



    I look forward to hearing from you. Should you have any further questions please do not hesitate to ask

    Read More

    Answer

    Thank you for contacting us.

    The MPC-11 myeloma is desribed in the following paper:

    J Exp Med. 1970 Mar 1;131(3):515-41.
    Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. Laskov R, Scharff MD. PMID:4189836


    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2138817/pdf/515.pdf


    My understanding is that the MPC-11 isotype control is not in fact a hybridoma but is simply a myeloma that secretes full-length IgG2b with no known specificity.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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