Mouse IgG2b kappa [MPC-11] (PE/Cy5®) - Isotype Control (ab239465)

Overview

  • Product name

    Mouse IgG2b kappa [MPC-11] (PE/Cy5®) - Isotype Control
  • Description

    Mouse IgG2b kappa [MPC-11] (PE/Cy5®) - Isotype Control
  • Conjugation

    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Specificity

    ab239465 reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse and rat tissues.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

  • Immunogen

    Chemical/ Small Molecule. KLH-coupled trinitrophenol.

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.0975% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    Purified antibody is conjugated with PE/Cy5® under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • Clonality

    Monoclonal
  • Clone number

    MPC-11
  • Isotype

    IgG2b
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab239465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab239465 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

Images

  • Example of non-specific ab239465 signal on human peripheral blood; surface staining, 1 μg/ml.

References

ab239465 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Questions for ab239465.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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