Overview

  • Product name
    Mouse IgG2b [PLPV219] - Isotype Control
    See all Mouse isotype controls
  • Specificity
    ab91366 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, ab91366 does not react with any antigen of Human or animal cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Vegetal protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    PLPV219
  • Isotype
    IgG2b
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells. ab91366 is a negative isotype control

Images

  • Overlay histogram showing HeLa cells stained with ab54944 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54944, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:
  • Cao Y  et al. ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression. Nat Commun 10:1280 (2019). Read more (PubMed: 30894532) »
  • Guo HH  et al. Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases. Nat Commun 10:1981 (2019). Read more (PubMed: 31040273) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. However you would have to use a mouse IgG1 as a control as the primary antibody (ab58137) has the isotype IgG1. I suggest isotype control antibody, mouse IgG1 [ICIGG1] ab91353 (www.abcam.com/ab91353).
Regarding PPAR-gamma ab, we do not have any antibodies which have been tested in flow cytometry.
However, if you would be willing to test one of these antibodies and providing us with your results, whether positive or negative, I could give you a discount.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
If you wish to test the antibody you suggested, ab41928, you could use the same secondary antibody but you would need a mouse IgG2a as a control. I suggest ab91361 as an isotype control (www.abcam.com/ab91361).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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https://www.abcam.com/abreviews

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