Mouse IgG2c ELISA Kit (ab157720)

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Overall				< 10%

Inter-assay
Sample	n	Mean	SD	CV%
Overall				< 10%

Recovery

> 85 %

Sample specific recovery
Sample type	Average %	Range
Serum	> 85	% - %

Assay duration

Multiple steps standard assay
Species reactivity

Reacts with: Mouse
Product overview

Abcam’s IgG2c Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG2c in mouse samples.
In this assay the IgG2c present in samples reacts with the anti-IgG2c antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG2c antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG2c. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG2c in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG2c in the test sample. The quantity of IgG2c in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Tested applications

Suitable for: Sandwich ELISAmore details
Platform

Microplate

Components	1 x 96 tests
100X HRP-conjugated anti-mouse IgG2c antibody	1 x 150µl
20X Wash Buffer Concentrate	1 x 50ml
5X Diluent Concentrate	1 x 50ml
Chromogen Substrate Solution	1 x 12ml
Mouse IgG2c Calibrator (lyophilized)	1 vial
Mouse IgG2c ELISA Microplate	1 unit
Stop Solution	1 x 12ml

Research areas
Relevance

There are four IgG subclasses (IgG1, 2, 3 and 4) in Humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria.
Alternative names
- Ig gamma-2 chain C region

Our Abpromise guarantee covers the use of ab157720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Sandwich ELISA		Use at an assay dependent concentration.

Standard Curve

Representative standard curve using ab157720 IgG2c Mouse ELISA Kit

Ma J et al. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation. EBioMedicine 22:143-154 (2017). Read more (PubMed: 28711483) »

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