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    mouse-igg2c-elisa-kit-ab157720.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Mouse IgG2c ELISA Kit (ab157720)

  • Datasheet
  • SDS
  • Protocol Booklet
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Standard Curve

    Key features and details

    • Sensitivity: 0.38 ng/ml
    • Range: 3.13 ng/ml - 200 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    Overview

    • Product name

      Mouse IgG2c ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.38 ng/ml
    • Range

      3.13 ng/ml - 200 ng/ml
    • Recovery

      > 85 %

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      Abcam’s IgG2c Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG2c in mouse samples.

      In this assay the IgG2c present in samples reacts with the anti-IgG2c antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG2c antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG2c. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG2c in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG2c in the test sample. The quantity of IgG2c in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X HRP-conjugated anti-mouse IgG2c antibody 1 x 150µl
      20X Wash Buffer Concentrate 1 x 50ml
      5X Diluent Concentrate 1 x 50ml
      Chromogen Substrate Solution 1 x 12ml
      Mouse IgG2c Calibrator (lyophilized) 1 vial
      Mouse IgG2c ELISA Microplate 1 unit
      Stop Solution 1 x 12ml
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgG
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Immunoglobulins ELISA kits
    • Relevance

      There are four IgG subclasses (IgG1, 2, 3 and 4) in Humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria.
    • Alternative names

      • Ig gamma-2 chain C region

    Images

    • Standard Curve
      Standard Curve
      Representative standard curve using ab157720 IgG2c Mouse ELISA Kit

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab157720? Please let us know so that we can cite the reference in this datasheet.

    ab157720 has been referenced in 1 publication.

    • Ma J  et al. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation. EBioMedicine 22:143-154 (2017). PubMed: 28711483

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