Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)

Overview

  • Product name
    Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, IHC-P, IHC-Frmore details
  • Immunogen

    Full length protein corresponding to Mouse conjugated to Keyhole Limpet Haemocyanin (KLH).

  • General notes

    ab18394 reacts with KLH. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Isotype control notes
    Ab18394 reacts with KLH. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues.
  • Clonality
    Monoclonal
  • Clone number
    MG3-35
  • Isotype
    IgG3
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab18394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

References

This product has been referenced in:
  • Mitchell J & Sullam PM Streptococcus mitis phage-encoded adhesins mediate attachment to {alpha}2-8-linked sialic acid residues on platelet membrane gangliosides. Infect Immun 77:3485-90 (2009). Read more (PubMed: 19506011) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Q&A

Answer

Thank you for contacting Abcam.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly.

For ab18394, we recommened that you store the product at 4C

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=popular_protocols).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for contacting Abcam regarding ab18394.


Attached please find the document you requested.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you for contacting us.

I did read over the specifications for dynabeads and I did not realize that they claim to have strong binding with IgG3 as most protein A beads have a weak binding with that particular isotype. However, because the primary antibody is IgG2b a correct isotype control will be anti-Mouse IgG2b.

Any isotype control should exhibit a typical banding of the heavy chain at ˜55kDa and of the light chain at ˜25kDa if the antibody has been reduced/denatured. Would it be possible to send the full membrane image with ladder as I was wondering if the light chain is present?

As always, this product is covered by our Abpromise guarantee.
If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet.

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Answer

Thank you for contacting us and thank you for providing the protocol and image.
After reviewing the data presented I would like to suggest a few changes that may help improve your results.

Mouse IgG3 does not bind well to beads for IP. To overcome this we recommend that when using an IgG3 antibody that you incubate for a longer period and use higher concentration. As your primary antibody is IgG2b, which binds more strongly to protein A beads, a correct isotype control will be anti-Mouse IgG2b. We do offer this product, ab18421. I have placed the link below.

https://www.abcam.com/Mouse-IgG2b-kappa-monoclonal-MG2b-57-Isotype-control-ab18421.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. The concentration of this product is 0.500 mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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