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Thank you for your reply. First of all, kindly advise the pattern of the run that the customer should observe with the isotype control. Secondly, please read the attached correspondence regarding the compatibility of ab18394 with IP and Invitrogen's Dynabeads protein A #100.01D. Please clarify. Thanks in advance for your assistance and reply.
Asked on Mar 12 2012
Thank you for contacting us.
I did read over the specifications for dynabeads and I did not realize that they claim to have strong binding with IgG3 as most protein A beads have a weak binding with that particular isotype. However, because the primary antibody is IgG2b a correct isotype control will be anti-Mouse IgG2b.
Any isotype control should exhibit a typical banding of the heavy chain at ˜55kDa and of the light chain at ˜25kDa if the antibody has been reduced/denatured. Would it be possible to send the full membrane image with ladder as I was wondering if the light chain is present?
As always, this product is covered by our Abpromise guarantee.
If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet.
Answered on Mar 12 2012